Antibiotic Susceptibility Testing (Agar Disk Diffusion and Agar Dilution) of Clinical Isolates of Corynebacterium jeikeium

• Published in: Chemotherapy (Basel) Vol. 44; no. 4; pp. 230 - 237
• Main Authors: Traub, Walter H., Geipel, Udo, Leonhard, Birgit, Bauer, Dierk
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.07.1998; S. Karger AG
• Subjects: Agar; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Corynebacterium - drug effects; Culture Media; Drug Resistance, Microbial; Enterococcus - drug effects; Evaluation Studies as Topic; Humans; Medical sciences; Microbial Sensitivity Tests; Microbiological Techniques; Microbiology; Pharmacology. Drug treatments; Wilkins-Chalgren agar; Antibiotic susceptibility; Mueller-Hinton agar; Agar disk diffusion (Bauer-Kirby) test; Agar dilution test; Minimal inhibitory concentrations; Corynebacterium jeikeium; Human; Antibiotic; Corynebacterium; Sensitivity resistance; Drug susceptibility test; Agar; Bacteria; Actinomycetes; Corynebacteriaceae; In vitro; Clinical isolate
• ISSN: 0009-3157; 1421-9794
• DOI: 10.1159/000007119

Thirty-three clinical isolates of Corynebacterium jeikeium were examined for susceptibility to 27 antimicrobial drugs with the agar dilution test. Sheep-blood-supplemented Mueller-Hinton agar performed better than Wilkins-Chalgren agar. Disk susceptibility (Bauer-Kirby) tests were carried out in parallel with 24 of the chemotherapeutic agents. All isolates were susceptible to teicoplanin and vancomycin. All isolates resisted fosfomycin, mupirocin, and trimethoprim-sulfamethoxazole. The isolates varied in susceptibility to ciprofloxacin, doxycycline, fusidic acid, ofloxacin, and tetracycline; most were susceptible to rifampin. Surprisingly few discrepancies between agar dilution and disk diffusion tests were encountered when utilizing NCCLS interpretive criteria currently valid for enterococcal isolates.