Alpha-lipoic acid and coenzyme Q10 combination ameliorates experimental diabetic neuropathy by modulating oxidative stress and apoptosis

• Published in: Life sciences (1973) Vol. 216; pp. 101 - 110
• Main Authors: Sadeghiyan Galeshkalami, Nasrin, Abdollahi, Mohammad, Najafi, Rezvan, Baeeri, Maryam, Jamshidzade, Akram, Falak, Reza, Davoodzadeh Gholami, Mohammad, Hassanzadeh, Gholamreza, Mokhtari, Tahmineh, Hassani, Shokoufeh, Rahimifard, Mahban, Hosseini, Asieh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.01.2019; Elsevier BV
• Subjects: Alpha-lipoic acid; Apoptosis; ATP; Biomarkers; Caspase; Caspase-3; Coenzyme Q10; Degeneration; Diabetes; Diabetes mellitus; Diabetic neuropathy; Dorsal root ganglia; Dorsal root ganglion neurons; Enzyme-linked immunosorbent assay; Lipoic acid; Mitochondrial uncoupling protein 2; Neurons; Neuroprotection; Oxidative stress; Proteins; Reactive oxygen species; Oxidative stress; Diabetic neuropathy; Coenzyme Q10; Dorsal root ganglion neurons; Alpha-lipoic acid; Apoptosis
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2018.10.055

Diabetic neuropathy (DN) is the most common complication of diabetes. Neuroprotective effects of alpha lipoic acid (ALA) and coenzyme Q10 (CoQ10) has been previously shown in DN, but underlying mechanisms involved not been exactly found. The present study explored the neuroprotective effects of ALA and Q10 combination in experimental DN by ameliorating oxidative stress and apoptosis. We investigated the effects of CoQ10 (10 mg/kg, orally, five weeks) and/or ALA (100 mg/kg, orally, five weeks) in STZ (45 mg/kg, i.p.)- induced DN in rats. After treatments motor function, oxidative stress biomarkers, ATP levels, expression of caspase 3 and UCP2 proteins were assessed by open-field, biochemical and ELISA methods and Western blot analysis. Dorsal root ganglion (DRG) neurons were histologically examined using H&E staining method. ALA and/or CoQ10 treatment significantly (p < 0.05) attenuated DN – induced motor function deficiency by modulating distance moved and velocity. ALA and/or CoQ10 treatment dramatically suppressed DN – induced oxidative stress which was associated with decrease in LPO and ROS and increase in GSH and TAC in DRG neurons. ALA and/or CoQ10 was proved to prevent apoptosis and degeneration of DRG neurons, which appears to be mediated by regulating the expression of caspase 3 and UCP2 proteins, inducing ATP and improving DN–induced changes in DRG neurons. We found maximum effectiveness with ALA and CoQ10 combination on mentioned factors. These results provide a possible basis of the underlying mechanism for application of ALA and CoQ10 combination in treatment of DN.