Monocarboxylate Transporter 1 (MCT1) Mediates Succinate Export in the Retina

• Published in: Investigative ophthalmology & visual science Vol. 63; no. 4; p. 1
• Main Authors: Bisbach, Celia M, Hass, Daniel T, Thomas, Eric D, Cherry, Timothy J, Hurley, James B
• Format: Journal Article
• Language: English
• Published: United States The Association for Research in Vision and Ophthalmology 01.04.2022
• Subjects: Membrane Transport Proteins; Retina; Retinal Pigment Epithelium; Succinates; Succinic Acid
• ISSN: 1552-5783; 0146-0404; 1552-5783
• DOI: 10.1167/iovs.63.4.1

Succinate is exported by the retina and imported by eyecup tissue. The transporters mediating this process have not yet been identified. Recent studies showed that monocarboxylate transporter 1 (MCT1) can transport succinate across plasma membranes in cardiac and skeletal muscle. Retina and retinal pigment epithelium (RPE) both express multiple MCT isoforms including MCT1. We tested the hypothesis that MCTs facilitate retinal succinate export and RPE succinate import. We assessed retinal succinate export and eyecup succinate import in short-term ex vivo culture using gas chromatography-mass spectrometry. We tested the dependence of succinate export and import on pH, proton ionophores, conventional MCT substrates, and the MCT inhibitors AZD3965, AR-C155858, and diclofenac. Succinate exits retinal tissue through MCT1 but does not enter the RPE through MCT1 or any other MCT. Intracellular succinate levels are a contributing factor that determines if an MCT1-expressing tissue will export succinate. MCT1 facilitates export of succinate from retinas. An unidentified, non-MCT transporter facilitates import of succinate into RPE.