Association of common IL-10 promoter gene variants with the susceptibility to head and neck cancer in Tunisia

• Published in: Turkish journal of medical sciences Vol. 49; no. 1; pp. 123 - 128
• Main Authors: Maknı, Lamia, Ben Hamda, Cherif, Al-ansarı, Abrar, Souıaı, Oussema, Gazouanı, Ezzedine, Mezlını, Amel, Almawı, Wassim, Yacoubı Loueslatı, Besma
• Format: Journal Article
• Language: English
• Published: Turkey The Scientific and Technological Research Council of Turkey 11.02.2019
• Subjects: Head and neck cancer; laryngeal cancer; nasopharyngeal cancer; interleukin-10; Tunisia
• ISSN: 1300-0144; 1303-6165
• DOI: 10.3906/sag-1805-21

We investigated the association of three IL-10 promoter single-nucleotide polymorphisms and altered IL-10 plasma levels with the risk of head and neck cancer (HNC). Study subjects comprised 194 HNC patients [137 nasopharyngeal cancer (NPC) and 57 laryngeal cancer (LC)], and 263 healthy controls. Genotyping of rs1800896 (-1082A>G), rs1800871 (-819C>T), and rs1800872 (-592A>C) IL-10 variants was performed by real-time PCR; IL-10 levels were measured by enzyme amplified immuno sensitivity assay (EAISA). Study subjects comprised 194 HNC patients [137 nasopharyngeal cancer (NPC) and 57 laryngeal cancer (LC)], and 263 healthy controls. Genotyping of rs1800896 (-1082A>G), rs1800871 (-819C>T), and rs1800872 (-592A>C) IL-10 variants was performed by real-time PCR; IL-10 levels were measured by enzyme amplified immuno sensitivity assay (EAISA). Our results demonstrate that IL-10-1082, IL-10-819, and IL-10-592 variants, and haplotypes GC and GT constitute biomarkers for early detection of HNC, especially NPC subtype. IL-10 -819T/C and TA haplotype may be used as biomarkers for early detection of LC.