Lab-on-nanopaper: An optical sensing bioplatform based on curcumin embedded in bacterial nanocellulose as an albumin assay kit

• Published in: Analytica chimica acta Vol. 1070; pp. 104 - 111
• Main Authors: Naghdi, Tina, Golmohammadi, Hamed, Vosough, Maryam, Atashi, Mojgan, Saeedi, Iman, Maghsoudi, Mohammad Taghi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06.09.2019; Elsevier BV
• Subjects: Albumin; Bacteria - chemistry; Bacterial cellulose nanopaper; Biodegradability; Biodegradation; Blood; Cellulose; Cellulose - chemistry; Curcumin; Curcumin - chemistry; Detection; Embedding; Glass substrates; Human serum albumin; Humans; Hydrophobicity; NAD; Nanostructures - chemistry; Optical properties; Optical sensor; Porosity; Serum albumin; Serum Albumin, Human - analysis; Smartphones; Albumin; Optical sensor; Curcumin; Bacterial cellulose nanopaper
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2019.04.037

Herein, we introduce a nanopaper-based analytical device (NAD) or “lab-on-nanopaper” device for visual sensing of human serum albumin (HSA) in human blood serums, which relies on embedding of curcumin within transparent bacterial cellulose (BC) nanopaper. BC nanopaper is an appropriate candidate to be an excellent platform for the development of optical (bio)sensors due to having exceptional properties such as optical transparency, high flexibility, porosity, biodegradability, and printability. The hydrophilic test zones were created on the fabricated bioplatform through creating the hydrophobic walls via laser printing technology. The color changes of curcumin embedded in BC nanopaper (CEBC) due to the inhibitory effect of HSA on the curcumin degradation in alkaline solutions, which can be monitored visually (naked eye/Smartphone camera) or spectroscopically using a spectrophotometer, were linearly proportional to the HSA concentration in the range of 10–300 μM and 25–400 μM, respectively. The developed NAD/CEBC as a novel albumin assay kit was successfully utilized to the determination of HSA in human blood serum samples with satisfactory results. Building upon the fascinating features of BC nanopaper as a very promising bioplatform in optical (bio)sensing applications we are confident “lab-on-nanopaper” devices/NADs, which take the advantages of the nanopaper and also meet the ASSURED criteria, could be considered as a new generation of optical (bio)sensing platforms that are currently based on paper, glass or plastic substrates. [Display omitted] •A nanopaper-based analytical device (NAD) was fabricated via embedding of curcumin in bacterial cellulose (BC) nanopaper.•The fabricated curcumin embedded in BC nanopaper (CEBC) was exploited as an albumin assay kit with satisfactory results.•The result of the developed NAD was in good agreement with that obtained by a clinical routine-method for HSA detection.•The developed “lab-on-nanopaper” devices could be considered as a new generation of optical (bio)sensing platforms.