Different proteome pattern of epidermal growth factor receptor–positive colorectal cancer cell lines that are responsive and nonresponsive to C225 antibody treatment
The monoclonal antibody C225 directed against the epidermal growth factor receptor (EGFR) blocks downstream mitogenic signaling and is effective in patients with advanced colorectal cancer. Clinical data, however, suggest the presence of primary and secondary resistance mechanisms that are hardly un...
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Published in | Molecular cancer therapeutics Vol. 3; no. 12; pp. 1551 - 1558 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Association for Cancer Research
01.12.2004
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Subjects | |
Online Access | Get full text |
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Summary: | The monoclonal antibody C225 directed against the epidermal growth factor receptor (EGFR) blocks downstream mitogenic signaling
and is effective in patients with advanced colorectal cancer. Clinical data, however, suggest the presence of primary and
secondary resistance mechanisms that are hardly understood. To define proteins involved in EGFR-triggered growth regulation
and potential resistance mechanisms, we characterized the proteome profile of two colorectal cancer cell lines with a high
expression of functional EGFR but a different response to treatment with C225. In Caco-2 and HRT-18, a complete saturation
of EGFR was achieved after incubation with C225; whereas Caco-2 showed inhibition of proliferation, growth of HRT-18 was not
suppressed. Using two-dimensional electrophoresis and subsequent mass spectrometry, we identified 14 proteins differentially
expressed in both cell lines. All proteins are involved in metabolic pathways and malignant growth. Expression of enzymes
such as ubiquitin carboxyl-terminal hydrolase isozyme 1, glutathione S -transferase P, and chloride intracellular channel protein 1 does not seem to interfere with the antiproliferative effect
of anti-EGFR antibody. On the other hand, expression of proteins such as fatty acid binding protein and heat shock protein
27 might constitute strong antiapoptotic effects contributing to the nonresponse of HRT-18 to C225 treatment. Proteome-based
investigations can help us better understand the complex protein interactions involved in EGFR signaling and its blockage
by therapeutic monoclonal antibodies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1535-7163 1538-8514 |
DOI: | 10.1158/1535-7163.1551.3.12 |