Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq

• Published in: Developmental biology Vol. 489; pp. 146 - 160
• Main Authors: Naraine, Ravindra, Iegorova, Viktoriia, Abaffy, Pavel, Franek, Roman, Soukup, Vladimír, Psenicka, Martin, Sindelka, Radek
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.09.2022
• Subjects: Amphibians; Egg; evo devo; Fishes; RNA localization; TOMO-Seq; RNA localization; Egg; TOMO-Seq; Amphibians; evo devo; Fishes
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2022.06.013

Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and establishment of many biological processes, cell types and the body plan. However, our knowledge about evolutionary conservation of localized transcripts is still limited to a few models. Our goal was to compare localization profiles along the animal-vegetal axis of mature eggs from four vertebrate models, two amphibians (Xenopus laevis, Ambystoma mexicanum) and two fishes (Acipenser ruthenus, Danio rerio) using the spatial expression method called TOMO-Seq. We revealed that RNAs of many known important transcripts such as germ layer determinants, germ plasm factors and members of key signalling pathways, are localized in completely different profiles among the models. It was also observed that there was a poor correlation between the vegetally localized transcripts but a relatively good correlation between the animally localized transcripts. These findings indicate that the regulation of embryonic development within the animal kingdom is highly diverse and cannot be deduced based on a single model. [Display omitted] •Copious amounts of asymmetrical genes within the egg of amphibians and fishes.•Maternal gene localization appears species specific.•Limited level of conservation of vegetal genes between species.•Majority of genes are at the animal pole.•High level of conservation of motifs within the vegetal genes of different species.