Quantitative micro determination of alcohols as esters of pyruvic acid 2,6-dinitrophenylhydrazone

• Published in: Analytical biochemistry Vol. 38; no. 1; pp. 148 - 157
• Main Author: Schwartz, Daniel P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.1970
• Subjects: Alcohols - analysis; alternative farming; Colorimetry; Esters; Fatty Acids; Methods; Microchemistry; organic production; Phenylhydrazines; Pyruvates

A colorimetric procedure is described for the quantitative determination of monohydric alcohols at the micromole level. The method is based on the acylation of the hydroxyl group with pyruvic acid chloride 2,6-dinitrophenylhydrazone in the presence of triethylenediamine and subsequent isolation of t...