Quantitative micro determination of alcohols as esters of pyruvic acid 2,6-dinitrophenylhydrazone

A colorimetric procedure is described for the quantitative determination of monohydric alcohols at the micromole level. The method is based on the acylation of the hydroxyl group with pyruvic acid chloride 2,6-dinitrophenylhydrazone in the presence of triethylenediamine and subsequent isolation of t...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 38; no. 1; pp. 148 - 157
Main Author Schwartz, Daniel P.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1970
Subjects
Alcohols - analysis
alternative farming
Colorimetry
Esters
Fatty Acids
Methods
Microchemistry
organic production
Phenylhydrazines
Pyruvates
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Summary:A colorimetric procedure is described for the quantitative determination of monohydric alcohols at the micromole level. The method is based on the acylation of the hydroxyl group with pyruvic acid chloride 2,6-dinitrophenylhydrazone in the presence of triethylenediamine and subsequent isolation of the derivative on alumina. The reaction is complete at room temperature upon addition of the base to the dried solution of the alcohol and acid chloride. Tertiary alcohols are quantitatively acylated as readily as primary and secondary alcohols. The procedure is applicable in both a small volume (1.8 ml) and a relatively large volume (25 ml) of benzene. Fatty acids inhibit the reaction.
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(70)90165-X