Quantitative micro determination of alcohols as esters of pyruvic acid 2,6-dinitrophenylhydrazone
A colorimetric procedure is described for the quantitative determination of monohydric alcohols at the micromole level. The method is based on the acylation of the hydroxyl group with pyruvic acid chloride 2,6-dinitrophenylhydrazone in the presence of triethylenediamine and subsequent isolation of t...
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| Published in | Analytical biochemistry Vol. 38; no. 1; pp. 148 - 157 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
01.01.1970
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| Subjects | |
| Online Access | Get full text |
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| Abstract | A colorimetric procedure is described for the quantitative determination of monohydric alcohols at the micromole level. The method is based on the acylation of the hydroxyl group with pyruvic acid chloride 2,6-dinitrophenylhydrazone in the presence of triethylenediamine and subsequent isolation of the derivative on alumina. The reaction is complete at room temperature upon addition of the base to the dried solution of the alcohol and acid chloride. Tertiary alcohols are quantitatively acylated as readily as primary and secondary alcohols. The procedure is applicable in both a small volume (1.8 ml) and a relatively large volume (25 ml) of benzene. Fatty acids inhibit the reaction. |
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| AbstractList | A colorimetric procedure is described for the quantitative determination of monohydric alcohols at the micromole level. The method is based on the acylation of the hydroxyl group with pyruvic acid chloride 2,6-dinitrophenylhydrazone in the presence of triethylenediamine and subsequent isolation of the derivative on alumina. The reaction is complete at room temperature upon addition of the base to the dried solution of the alcohol and acid chloride. Tertiary alcohols are quantitatively acylated as readily as primary and secondary alcohols. The procedure is applicable in both a small volume (1.8 ml) and a relatively large volume (25 ml) of benzene. Fatty acids inhibit the reaction. |
| Author | Schwartz, Daniel P. |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/5478236$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/0026-265X(67)90041-0 10.1016/0026-265X(68)90068-4 10.1021/ac60210a062 10.1021/jo01364a003 10.1021/ac60176a050 10.1016/0026-265X(68)90107-0 10.1016/0026-265X(67)90040-9 10.1016/0039-9140(66)80242-4 10.1016/0026-265X(67)90003-3 10.1016/0026-265X(66)90114-7 10.1021/ac60163a042 10.1016/0026-265X(68)90020-9 10.1016/0026-265X(68)90019-2 10.1016/S0003-2670(01)80607-3 |
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| References | Johnson, Critchfield (BIB4) 1960; 32 Schwartz, Brewington (BIB5) 1966; 11 Schwartz, Brewington (BIB7) 1968; 13 Schwartz, Shamey, Brewington, Parks (BIB14) 1968; 13 Schenk, Wines, Mojzis (BIB16) 1964; 36 Schwartz, Brewington (BIB10) 1967; 12 Fieser (BIB12) 1964 Maienthal, Franklin (BIB13) 1955; 20 Mathur (BIB3) 1966; 13 Bassette, Brewington, Schwartz (BIB11) 1968; 13 Robinson, Cundiff, Markunas (BIB15) 1961; 33 Schwartz, Brewington, Shamey (BIB8) 1967; 12 Belcher, Dryhurst, Macdonald (BIB1) 1967; 38 Dryhurst (BIB2) 1964; 40 Schwartz, Brewington (BIB6) 1967; 12 Schwartz, Brewington (BIB9) 1968; 13 Belcher (10.1016/0003-2697(70)90165-X_BIB1) 1967; 38 Mathur (10.1016/0003-2697(70)90165-X_BIB3) 1966; 13 Maienthal (10.1016/0003-2697(70)90165-X_BIB13) 1955; 20 Schwartz (10.1016/0003-2697(70)90165-X_BIB5) 1966; 11 Schwartz (10.1016/0003-2697(70)90165-X_BIB9) 1968; 13 Schwartz (10.1016/0003-2697(70)90165-X_BIB10) 1967; 12 Dryhurst (10.1016/0003-2697(70)90165-X_BIB2) 1964; 40 Schwartz (10.1016/0003-2697(70)90165-X_BIB7) 1968; 13 Robinson (10.1016/0003-2697(70)90165-X_BIB15) 1961; 33 Schenk (10.1016/0003-2697(70)90165-X_BIB16) 1964; 36 Bassette (10.1016/0003-2697(70)90165-X_BIB11) 1968; 13 Schwartz (10.1016/0003-2697(70)90165-X_BIB8) 1967; 12 Johnson (10.1016/0003-2697(70)90165-X_BIB4) 1960; 32 Schwartz (10.1016/0003-2697(70)90165-X_BIB6) 1967; 12 Schwartz (10.1016/0003-2697(70)90165-X_BIB14) 1968; 13 Fieser (10.1016/0003-2697(70)90165-X_BIB12) 1964 |
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| SubjectTerms | Alcohols - analysis alternative farming Colorimetry Esters Fatty Acids Methods Microchemistry organic production Phenylhydrazines Pyruvates |
| Title | Quantitative micro determination of alcohols as esters of pyruvic acid 2,6-dinitrophenylhydrazone |
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