LOCATE enzyme-linked immunosorbent assay for detection of Salmonella in food: collaborative study

• Published in: Journal of AOAC International Vol. 81; no. 2; p. 419
• Main Authors: Gangar, V. (Silliker Laboratories Group, Inc., South Holland, IL.), Curiale, M.S, D'onorio, A, Donnelly, C, Dunnigan, P
• Format: Journal Article
• Language: English
• Published: England 01.03.1998
• Subjects: ALIMENTOS; ANALYTICAL METHODS; Animals; BIOLOGICAL CONTAMINATION; BLACK PEPPER; Cacao - chemistry; Cacao - microbiology; CARNE DE PAVO; CHOCOLAT; CHOCOLATE; CONTAMINACION; CONTAMINACION BIOLOGICA; CONTAMINATION; CONTAMINATION BIOLOGIQUE; DRIED MILK; EGGS; Eggs - analysis; Eggs - microbiology; ELISA; ENSAYO; Enzyme-Linked Immunosorbent Assay; EVALUACION; EVALUATION; Flour - analysis; Flour - microbiology; Food Contamination - analysis; Food Microbiology; FOODS; HUEVOS; Indicators and Reagents; LAIT DESHYDRATE; LECHE EN POLVO; Meat - analysis; Meat - microbiology; MICROBIAL CONTAMINATION; Milk - chemistry; Milk - microbiology; OEUF; PEPPER; PIMIENTA; POIVRE; PRODUIT ALIMENTAIRE; RAPID METHODS; RAW FOODS; Reproducibility of Results; SALMONELLA; Salmonella - chemistry; SEROTIPOS; SEROTYPE; SEROTYPES; Serotyping; SOYBEAN FLOUR; SOYBEAN MEAL; Spices - analysis; Spices - microbiology; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; TEST ELISA; TEST PROCEDURE; TESTAGE; TESTING; TORTAS DE SOJA; TOURTEAU DE SOJA; TURKEY MEAT; Turkeys; VIANDE DE DINDE
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/81.2.419

A collaborative study was performed in 27 laboratories to validate the enzyme-linked immunosorbent procedure LOCATE for rapid detection of Salmonella in foods. Results were read visually and with a microfilter plate reader. The LOCATE method was compared with the Bacteriological Analytical Manual (BAM)/AOAC INTERNATIONAL culture method for detecting Salmonella in 6 foods: milk chocolate, nonfat dry milk, dried whole egg, soy flour, ground black pepper, and ground raw turkey. Two foods--dried whole egg and black pepper--required repeat rounds because insufficient data sets were produced initially (AOAC INTERNATIONAL stipulates a minimum of 15 sets per food type). Each laboratory tested one or more of the 6 foods. A total of 1439 samples were analyzed, and no significant differences (P 0.05) were observed between LOCATE with either visual or reader detection and BAM/AOAC INTERNATIONAL results. The LOCATE screening method with visual or reader detection is recommended for Official First Action Approval