Porcine leukocyte 5- and 12-lipoxygenases are iron enzymes

• Published in: FEBS letters Vol. 287; no. 1; pp. 105 - 107
• Main Authors: Kroneck, Peter M.H., Cucurou, Christophe, Ullrich, Volker, Ueda, Natsuo, Suzuki, Hiroshi, Yoshimoto, Tanihiro, Matsuda, Satoshi, Yamamoto, Shozo
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.08.1991
• Subjects: 12-HPETE; 12-hydroperoxy-eicosatetraenoic acid; 9-HPOD; 9-hydroperoxyoctadecadienoic acid; Animals; Arachidonate 12-Lipoxygenase - analysis; Arachidonate 12-Lipoxygenase - chemistry; Arachidonate 5-Lipoxygenase - analysis; Arachidonate 5-Lipoxygenase - chemistry; DETAPAC; diethylenetriamine-pentaacetic acid; Electron paramagnetic resonance; Electron Spin Resonance Spectroscopy; EPR; EXAFS; extended X-ray absorption fine structure; Glycine max - enzymology; Iron - analysis; Iron enzyme: Lipoxygenase; Leukocytes - enzymology; Leukotrienes - pharmacology; Linoleic Acids - pharmacology; Lipoxygenase Inhibitors; Masoprocol - pharmacology; NDGA; nordihydroguaiaretic acid; Porcine leukocytes; Solanum tuberosum - enzymology; SP-HPLC; straight phase high performance liquid chromatography; Swine; DETAPAC; 9-HPOD; NDGA; 12-HPETE; EPR; Iron enzyme: Lipoxygenase; Electron paramagnetic resonance; SP-HPLC; EXAFS; Porcine leukocytes
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)80026-Y

5- and 12-lipoxygenases isolated from porcine leukocytes were investigated by electron paramagnetic resonance at X-band and atomic absorption spectroscopy. For comparison potato 5-lipoxygenase was studied under identical experimental conditions. All three lipoxygenases contained between 0.7 and 0.9 Fe atoms/enzyme molecule. As isolated, both mammalian enzymes exhibited a characteristic EPR signal at low magnetic field with a maximum at g =5.20 indicative of a high-spin ferric iron center. The signal was not affected by the oxidants 12-hydroperoxyeicosatetraenoic acid or arachidonic acid, nor was it affected by the reductant nordihydroguaiaretic acid. In the case of the potato enzyme an intense EPR signal with resonances at g =7.50, 6.39 and 5.84 was only observed after addition of an oxidant, such as 9-hydroperoxyoctadecadienoic acid.