Proteomic Analysis of the Response of Liangyoupeijiu (Super High-Yield Hybrid Rice) Seedlings to Cold Stress
Liangyoupeijiu is a super high‐yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4°C cold treatment, then extracted the leaf proteins. After 2‐D gel electro...
Saved in:
Published in | Journal of integrative plant biology Vol. 48; no. 8; pp. 945 - 951 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Melbourne, Australia
Blackwell Publishing Asia
01.08.2006
Graduate College of the Chinese Academy of Sciences, Beijing 100039, China%Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany,the Chinese Academy of Sciences, Beijing 100093, China Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany,the Chinese Academy of Sciences, Beijing 100093, China |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Liangyoupeijiu is a super high‐yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4°C cold treatment, then extracted the leaf proteins. After 2‐D gel electrophoresis separation and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry analysis, a series of differentially displayed proteins were identified. Some metabolism‐associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3‐phosphate dehydrogenase. The upregulated proteins included both stress‐resistance proteins such as nucleoside diphosphate kinase I and proteins that are negative for rice growth, such as FtsH‐like protein, plastid fusion and/or translocation factor (Pftf) and actin. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.
(Managing editor: Li‐Hui Zhao) |
---|---|
Bibliography: | ArticleID:JIPB319 istex:25C000979D7A78B183283E455560D698460C5DFF ark:/67375/WNG-QLT830C2-C Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2‐SW‐307) and the Knowledge Innovation Program of the Institute of Botany, the Chinese Academy of Sciences. |
ISSN: | 1672-9072 1744-7909 |
DOI: | 10.1111/j.1744-7909.2006.00319.x |