Comparison of the use of anionic and cationic surfactants for the separation of steroids based on MEKC and sweeping-MEKC modes
In attempts to improve the selectivity and sensitivity of steroid separation and to determine their migration order, a comparison of the use of anionic and cationic surfactants based on the MEKC and sweeping‐MEKC modes was made. A mixture of six steroids (progesterone, 17‐hydroxy progesterone, 11‐de...
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Published in | Electrophoresis Vol. 27; no. 5-6; pp. 1255 - 1262 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.03.2006
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | In attempts to improve the selectivity and sensitivity of steroid separation and to determine their migration order, a comparison of the use of anionic and cationic surfactants based on the MEKC and sweeping‐MEKC modes was made. A mixture of six steroids (progesterone, 17‐hydroxy progesterone, 11‐deoxycortisol, corticosterone, cortisone, and cortisol) could be separated and detected by means of the CE/UV‐absorption method. The order of migration time for these steroids was compared under various conditions, including acidic/alkaline buffers, anionic/cationic surfactants, and positive/negative applied voltage, causing the direction of the EOF and the migration of micelles to change. The major rules for generally predicting the migration order of steroids are summarized. The detection limits were significantly improved when the sweeping‐MEKC mode was applied. |
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Bibliography: | istex:D39CBF1EBEF77309C0D7A7A7C50A4A6AD156A34B ark:/67375/WNG-3DJHSJR6-V ArticleID:ELPS200500659 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200500659 |