Exploring Quaternized Hydroxyethylcellulose as Potential Gene Carriers

Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and res...

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Published inChinese journal of chemistry Vol. 30; no. 9; pp. 2212 - 2218
Main Author 丁菲 尤俊 翁小成 周金平 章晓联 周翔 张俐娜
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.09.2012
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
cationic cellulose
Deoxyribonucleic acid
DNA
DNase
gene carrier
Genes
Saccharides
transfection
动态光散射
基因载体
复合物
季铵化
结合能力
羟乙基纤维素
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ISSN1001-604X
1614-7065
DOI10.1002/cjoc.201200659

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Summary:Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter （DLS） results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfeetion abilities in comparison to the naked DNA. The cytotox- icities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low com- pared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as poten- tial gene delivery vectors.
Bibliography:31-1547/O6
cationic cellulose, DNA, gene carrier, transfection
Ding, Fei You, Jun Weng, Xiaocheng Zhou, Jinping Zhou, Xiang Zhang, Xiaolian Zhang, Lin（a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China b School of Medicine, Wuhan University, Hubei, Wuhani 430071, China c State Key Lab ofBioorganic ＆ Natural Product Chemistry, Shanghai Institute of Organic Chememistry, Shanghai 200032, China）
Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter （DLS） results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfeetion abilities in comparison to the naked DNA. The cytotox- icities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low com- pared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as poten- tial gene delivery vectors.
ark:/67375/WNG-GXX3MFRL-X
the National Natural Science Foundation of China - No. 90813031, 21072115, 30973605, 21272181
the National Basic Research Program of China (973 Program) - No. 2010CB732203, 2012CB720603
ArticleID:CJOC201200659
National Grand Program on Key Infectious Disease - No. 2012ZX10003-002
the Program for Changjiang Scholars and Innovative Research Team in University - No. IRT1030
istex:72A9F0C45B20A22AB1F7987DEA77D8D45F63F2C2
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.201200659