Proteomics-based Characterization of Protein Complexes from Human Pancreatic Cancer Cell Line

To develop an economical and feasible approach to probe protein complexes, differential centrifugation and three-dimensional polyacrylamide gel electrophoresis （PAGE） were performed to separate protein complexes from the cell lysate of human pancreatic cancer cell line, SW1990, followed by mass spec...

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Published inChinese journal of chemistry Vol. 29; no. 7; pp. 1548 - 1550
Main Author 王新立 陈国强 赵志云 王晓东 李智立
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.07.2011
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
Aquatic life
Invertebrates
mass spectrometry
Pancreatic cancer
pancreatic cancer cell line
proteasome
protein complex
Proteins
Proteomics
three dimensional polyacrylamide gel electrophoresis
细胞株
细胞裂解液
聚丙烯酰胺凝胶电泳
胰腺癌
蛋白复合物
蛋白质复合物
蛋白质组学
表征
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Summary:To develop an economical and feasible approach to probe protein complexes, differential centrifugation and three-dimensional polyacrylamide gel electrophoresis （PAGE） were performed to separate protein complexes from the cell lysate of human pancreatic cancer cell line, SW1990, followed by mass spectrometric identification. Four macromolecular protein complexes were separated and identified unambiguously.
Bibliography:31-1547/O6
three dimensional polyacrylamide gel electrophoresis, protein complex, proteasome, pancreatic cancercell line, mass spectrometry
To develop an economical and feasible approach to probe protein complexes, differential centrifugation and three-dimensional polyacrylamide gel electrophoresis （PAGE） were performed to separate protein complexes from the cell lysate of human pancreatic cancer cell line, SW1990, followed by mass spectrometric identification. Four macromolecular protein complexes were separated and identified unambiguously.
Wang, Xinli Chen, Guoqiang Zhao, Zhiyun Wang, Xiaodong Li, Zhili（Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China）
the National High-Tech R&D Program of China - No. 2006AA02Z154
istex:7A14B3EBCA74949D0D08888CE7D8F1BA6864CA8B
ArticleID:CJOC201180278
ark:/67375/WNG-LBHHKZ68-X
the National Natural Science Foundation of China - No. 21075137
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.201180278