Sperm ultrastructure in honeycomb (foam) oysters (Mollusca, Bivalvia, Gryphaeidae, Pycnodontinae): comparison with other Ostreoidea and taxonomic implications

• Published in: Invertebrate biology Vol. 134; no. 2; pp. 136 - 150
• Main Authors: Healy, John M, Mikkelsen, Paula M, Bieler, Rüdiger
• Format: Journal Article
• Language: English
• Published: Hoboken Allen Press 01.06.2015; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: bivalve; centrioles; combs (social insects); flagellum; foams; gametes; Gryphaeidae; marine fauna; mitochondria; Ostreidae; oysters; reproduction; spermatozoa; systematics; texture; ultrastructure
• ISSN: 1077-8306; 1744-7410
• DOI: 10.1111/ivb.12086

Sperm ultrastructural features of the honeycomb (foam) oysters Hyotissa hyotis, H. sinensis, and H. mcgintyi (Gryphaeidae) are described and compared with other Ostreoidea and more generally with other pteriomorphian Bivalvia. Spermatozoa of H. sinensis and H. mcgintyi (the type species of Parahyotissa Harry 1985) exhibit (1) a broad, low‐conical acrosomal vesicle; (2) subacrosomal material (very electron‐dense granular material and an almost electron‐lucent axial rod); (3) a spheroidal nucleus with a wide anterior invagination (filled with subacrosomal components); (4) a midpiece composed of four spherical mitochondria surrounding a pair of centrioles (rootlet associated with proximal centriole); and (5) a flagellum. Sperm of Hyotissa hyotis (type species of Hyotissa Stenzel 1971) differ markedly from those of H. sinensis and H. mcgintyi, in having (1) a conical acrosomal vesicle showing coarse granular texture anteriorly; (2) a very electron‐dense axial rod; (3) a barrel‐shaped nucleus with a long, narrow anterior invagination (filled with both subacrosomal components) and a basal invagination partly housing the proximal centriole; and (4) five midpiece mitochondria and no proximal centriolar rootlet. Results indicate that H. sinensis should be relocated to another genus, possibly a revised genus Parahyotissa, and also show that the sperm of H. sinensis and H. mcgintyi show many similarities to those of the Ostreidae, with the exception that the ‘axial rod’ component of the subacrosomal material is less electron‐dense than the surrounding substance (more dense in Ostreidae, as in H. hyotis). No family defining sperm features of the Gryphaeidae can be identified.