Resistance against pepper severe mosaic potyvirus in transgenic tobacco plants

• Published in: Journal of phytopathology Vol. 146; no. 7; pp. 315 - 319
• Main Authors: Rabinowicz, P.D, Bravo-Almonacid, F.F, Lampasona, S, Rodriguez, F, Gracia, O, Mentaberry, A.N
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.1998; Blackwell
• Subjects: Biological and medical sciences; Biotechnology; coat proteins; disease resistance; enzyme-linked immunosorbent assay; Fundamental and applied biological sciences. Psychology; gene expression; genes; Genetic engineering; genetic resistance; Genetic technics; Methods. Procedures. Technologies; Microbiology; Nicotiana tabacum; Pepper severe mosaic virus; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; RNA; Transgenic animals and transgenic plants; Transgenic plants; Virology; Plant pathogen; Stimulant plant; Nicotiana tabacum; Protein; Infection; Virus; Characterization; Resistance; Pepper severe mosaic virus; Dicotyledones; Viral disease; Angiospermae; Spermatophyta; Transgenic plant; Potyvirus; Solanaceae; Experimental plant; Capsid; Potyviridae
• ISSN: 0031-9481; 0931-1785; 1439-0434
• DOI: 10.1111/j.1439-0434.1998.tb04698.x

The coat protein gene of the pepper severe mosaic poty‐virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon challenge with PepSMV, R2 descendants from this line were monitored for virus accumulation for a period of 45 days. Virus titre was lower in plants of the line 232 than in control plants throughout the experiment. At 14 days post‐inoculation the transgenic plant line reached the maximum virus accumulation measured by ELISA. Those plants also showed a 1 week delay of virus accumulation by comparison with control plants. After reaching the peak at 14 days post‐inoculation the virus titre in line 232 decreased with respect to control plants, until the end of the experiment. These results are compatible with the presence of an RNA‐mediated resistance mechanism. Zusammenfassung Das Hüllproteingen des Pepper severe mosaic potyvirus wurde in Tabakpflanzen eingeführt, und mehrere transgene Linien wurden unter Gewächshausbedingungen hinsichtlich einer Virusresistenz geprüft. Die Linie 232 zeigte ein besonders hohes Resistenzniveau und wurde genauer untersucht. Nach Herausforderung mit PepSMV wurde die Virusakkumulation in R2‐Abkömmlingen dieser Linie über 45 Tage untersucht. Der Virustiter war in den Pflanzen der Linie 232 stets niedriger als in den Kontrollpflanzen, 14 Tage nach der Inokulation (14 dpi) war die maximale durch ELISA gemessene Virusakkumulation in der transgenen Pflanzenlinie erreicht. Im Vergleich zu den Kontrollpflanzen war die Virusakkumulation in den transgenen Pflanzen außerdem um 1 Woche verzögert, Nach Erreichen des 14‐dpi‐Peaks nahm der Virustiter in der Linie 232 relativ zu den KontroUpflanzen bis zum Ende des Expedments ab. Diese Ergebnisse sind mit der Existenz eines RNA‐vermittelten Resistenzmechanismus vereinbar.