Nanoparticle PCR: Nanogold-Assisted PCR with Enhanced Specificity

• Published in: Angewandte Chemie International Edition Vol. 44; no. 32; pp. 5100 - 5103
• Main Authors: Li, Haikuo, Huang, Jiehuan, Lv, Junhong, An, Hongjie, Zhang, Xiaodong, Zhang, Zhizhou, Fan, Chunhai, Hu, Jun
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 12.08.2005; WILEY‐VCH Verlag
• Subjects: DNA - analysis; DNA replication; gold; Gold - chemistry; Nanostructures - chemistry; nanotechnology; polymerase chain reaction; Polymerase Chain Reaction - methods; Sensitivity and Specificity

Nanogold may not glitter, but its presence significantly improves the specificity and yield of PCR reactions owing to the greater affinity of gold nanoparticles to single‐stranded DNA than to double‐stranded DNA which helps to reduce mispairing. Indeed, gel electrophoresis shows a single predominant...