Nanoparticle PCR: Nanogold-Assisted PCR with Enhanced Specificity

Nanogold may not glitter, but its presence significantly improves the specificity and yield of PCR reactions owing to the greater affinity of gold nanoparticles to single‐stranded DNA than to double‐stranded DNA which helps to reduce mispairing. Indeed, gel electrophoresis shows a single predominant...

Full description

Saved in:
Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 44; no. 32; pp. 5100 - 5103
Main Authors Li, Haikuo, Huang, Jiehuan, Lv, Junhong, An, Hongjie, Zhang, Xiaodong, Zhang, Zhizhou, Fan, Chunhai, Hu, Jun
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 12.08.2005
WILEY‐VCH Verlag
Subjects
DNA - analysis
DNA replication
gold
Gold - chemistry
Nanostructures - chemistry
nanotechnology
polymerase chain reaction
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Online AccessGet full text

Cover

More Information
Summary:Nanogold may not glitter, but its presence significantly improves the specificity and yield of PCR reactions owing to the greater affinity of gold nanoparticles to single‐stranded DNA than to double‐stranded DNA which helps to reduce mispairing. Indeed, gel electrophoresis shows a single predominant band for the target DNA obtained by this method, in contrast to streaking bands for products obtained by conventional PCR (lanes 1 and 2; see image).
Bibliography:This work was supported by the Chinese Academy of Sciences, the National Natural Science Foundation (10335070 and 20404016), the Ministry of Science and Technology of China (2002CCA00600, 2003A310A02, and 2003AA226011), the Shanghai Municipal Commission for Science and Technology (0452M068, 03DZ14025, and 02JC14029), and the Ministry of Personnel. We also thank Prof. Kevin Plaxco (UCSB) for helpful comments.
ark:/67375/WNG-2LRVKRGT-D
istex:56E08A55C882C67BD0E3AA7401F67DF0F7BB181F
ArticleID:ANIE200500403
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200500403