Supramolecular Structure of Self-Assembling Alamethicin Analog Studied by ESR and PELDOR

• Published in: Chemistry & biodiversity Vol. 4; no. 6; pp. 1275 - 1298
• Main Authors: Milov, Alexander D., Samoilova, Marina I., Tsvetkov, Yuri D., Jost, Micha, Peggion, Cristina, Formaggio, Fernando, Crisma, Marco, Toniolo, Claudio, Handgraaf, Jan-Willem, Raap, Jan
• Format: Journal Article
• Language: English
• Published: Zürich WILEY-VCH Verlag 01.06.2007; WILEY‐VCH Verlag
• Subjects: Alamethicin - chemistry; Anti-Bacterial Agents - chemistry; Electron Spin Resonance Spectroscopy - methods; ESR Spectroscopy; Ion channels; Membrane-penetrating peptides; Peptaibols; Peptides; Protein Conformation; Pulsed electron-electron double resonance (PELDOR)
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.200790110

Three analogs of alamethicin F50/5, labelled with the TOAC (=‘2,2,6,6‐tetramethylpiperidin‐1‐oxyl‐4‐amino‐4‐carboxylic acid’) spin label at positions 1 (Alm1), 8 (Alm8), and 16 (Alm16), resp., were studied by Electron‐Spin‐Resonance (ESR) and Pulsed Electron–Electron Double‐Resonance (PELDOR) techniques in solvents of different polarity to investigate the self‐assembly of amphipathic helical peptides in membrane‐mimicking environments. In polar solvents, alamethicin forms homogeneous solutions. In the weakly polar chloroform/toluene 1 : 1 mixture, however, this peptide forms aggregates that are detectable at 293 K by ESR in liquid solution, as well as by PELDOR in frozen, glassy solution at 77 K. In liquid solution, free alamethicin molecules and their aggregates show rotational‐mobility correlation times τr of 0.87 and 5.9 ns, resp. Based on these values and analysis of dipole–dipole interactions of the TOAC labels in the aggregates, as determined by PELDOR, the average number N of alamethicin molecules in the aggregates is estimated to be less than nine. A distance‐distribution function between spin labels in the supramolecular aggregate was obtained. This function exhibits two maxima: a broad one at a distance of 3.0 nm, and a wide one at a distance of ca. 7 nm. A molecular‐dynamics (MD)‐based model of the aggregate, consisting of two parallel tetramers, each composed of four molecules arranged in a ‘head‐to‐tail’ fashion, is proposed, accounting for the observed distances and their distribution.