Effects of a low‐intensity resistance exercise program on serum miR‐630, miR‐5703, and Fractalkine/CX3CL1 expressions in subjects with No exercise habits: A preliminary study

• Published in: Hepatology research Vol. 51; no. 7; pp. 823 - 833
• Main Authors: Hashida, Ryuki, Matsuse, Hiroo, Kawaguchi, Takumi, Yoshio, Sachiyo, Bekki, Masafumi, Iwanaga, Sohei, Sugimoto, Takahiro, Hara, Koji, Koya, Shunji, Hirota, Keisuke, Nakano, Dan, Tsutsumi, Tsubasa, Kanto, Tatsuya, Torimura, Takuji, Shiba, Naoto
• Format: Journal Article
• Language: English
• Published: Netherlands Wiley Subscription Services, Inc 01.07.2021
• Subjects: cytokine; Cytokines; Fractalkine; Genomes; Liver cancer; metabolic dysfunction‐associated fatty liver disease; microRNA; MicroRNAs; miRNA; myokine; physicalactivity; resistance training; Transforming growth factor-b; Wnt protein; cytokine; myokine; microRNA; resistance training; metabolic dysfunction-associated fatty liver disease; physical activity
• ISSN: 1386-6346; 1872-034X
• DOI: 10.1111/hepr.13670

Aims Exercise is effective for the prevention of liver cancer. Exercise exerts biological effects through the regulation of microRNAs (miRNAs) and cytokines/myokines. We aimed to investigate the effects of low‐intensity resistance exercise on serum miRNA and cytokine/myokine expressions in subjects with no exercise habits. Methods We enrolled seven male subjects with no exercise habits in this prospective before‐after study. All subjects performed a low‐intensity resistance exercise program (three metabolic equivalents, approximately 20 min/session). Serum miRNA expressions were evaluated using microarrays. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed miRNAs before and after exercise. Serum cytokine/myokine expressions were evaluated using a multiplex panel. Results All subjects completed the exercise program with no adverse events. In the microarray analysis, seven miRNAs showed a significant change between before and after exercise. Of these, microRNA (miR)‐630 and miR‐5703 showed a >1.5‐fold increase (miR‐630: 40.7 vs. 69.3 signal intensity, p = 0.0133; miR‐5703: 30.7 vs. 55.9 signal intensity, p = 0.0051). KEGG pathway enrichment analysis showed that miR‐630‐ and miR‐5703‐related genes were enriched in 37 and 5 pathways, including transforming growth factor‐beta and Wnt signaling pathways, respectively. In the multiplex analysis, 12 cytokines/myokines showed significant alteration after exercise compared to before exercise. Of these, fractalkine/CX3CL1 showed the most significant up‐regulation by exercise (94.5 vs. 109.1 pg/ml, p = 0.0017). Conclusions A low‐intensity resistance exercise program was associated with upregulation of serum miR‐630, miR‐5703, and fractalkine/CX3CL1 expressions in subjects with no exercise habits. Thus, even low‐intensity exercise may alter miRNA and cytokine/myokine expressions in humans.