cAMP enhanced aggregation of cells from early chick embryos

Whole chick embryos incubated for 24–36 hr were disaggregated with EDTA. The populations of single cells were incubated both in suspension and after being plated at various densities on agar blocks in a humid environment. In both cases aggregates formed. The aggregation was enhanced by cAMP and 3-is...

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Bibliographic Details
Published inDevelopmental biology Vol. 58; no. 2; pp. 394 - 401
Main Author Gingle, Alan R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.07.1977
Subjects
adenosine monophosphate
Animals
Cell Aggregation - drug effects
Chick Embryo - cytology
Cyclic AMP - pharmacology
Cyclic AMP - physiology
Phosphoric Diester Hydrolases - pharmacology
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Summary:Whole chick embryos incubated for 24–36 hr were disaggregated with EDTA. The populations of single cells were incubated both in suspension and after being plated at various densities on agar blocks in a humid environment. In both cases aggregates formed. The aggregation was enhanced by cAMP and 3-isobutyl-1-methylxanthine (IBMX; a phosphodiesterase inhibitor). The density of aggregates which formed on the agar blocks decreased sharply at a critical cell density, suggesting that aggregation was mediated by a relayed signal. The critical density was decreased by IBMX and increased by phosphodiesterase (PDE), suggesting that aggregation was mediated by a cyclic nucleotide, most probably cAMP. Evidence was obtained for the presence of an extracellular PDE.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0012-1606
1095-564X
DOI:10.1016/0012-1606(77)90100-2