Isolation of a putative ribosome inactivating protein from dried roots of Trichosanthes kirilowii used in Traditional Chinese Medicine

• Published in: Planta medica Vol. 70; no. 4; pp. 364 - 365
• Main Authors: Fei, X, Hill, M, Ma, X, Teng, L, Li, Q, Stockigt, J
• Format: Journal Article
• Language: English
• Published: Germany 01.04.2004
• Subjects: Amino Acid Sequence; amino acid sequences; consensus sequence; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - isolation & purification; Electrophoresis, Polyacrylamide Gel; high performance liquid chromatography; Humans; medicinal plants; Molecular Sequence Data; Oriental traditional medicine; Phytotherapy; plant extracts; plant proteins; Plant Proteins - analysis; Plant Proteins - isolation & purification; Plant Roots; Protein Synthesis Inhibitors - analysis; Protein Synthesis Inhibitors - isolation & purification; ribosome inactivating protein; ribosomes; roots; Trichosanthes; Trichosanthes kirilowii
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-2004-818950

A putative ribosome inactivating protein (RIP), named T 33, was isolated from dried roots of Trichosanthes kirilowii Maximovicz which are used in Traditional Chinese Medicine. After chromatographic enrichment a protein band with molecular mass of 33 kDa was found by SDS-PAGE. Partial amino acid sequences of this protein consisting of 8 - 33 amino acids reveal homology to highly conserved regions of different RIPs, but also indicate T 33 to be a novel type 2 RIP. Future heterologous expression of this protein will allow investigation of its biological activities in detail.