Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells
Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dor...
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Published in | Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 1; pp. 76 - 80 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.02.2014
The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China |
Subjects | |
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Abstract | Summary
In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG. |
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AbstractList | In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG. Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG. |
Author | Lin, Qiang Cai, Yang-ting Li, Hao-shen Chen, Hai-yun |
AuthorAffiliation | The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China |
AuthorAffiliation_xml | – name: The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China |
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Cites_doi | 10.1016/j.neuint.2006.10.010 10.1523/JNEUROSCI.6271-09.2010 10.1371/journal.pone.0030012 10.1074/jbc.M110.125336 10.1523/JNEUROSCI.4340-10.2010 10.1038/nn.2603 10.1126/science.1161566 |
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Keywords | controlled release microspheres neural stem cells PLLA/bpV(pic) dorsal root ganglion |
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PublicationTitle | Journal of Huazhong University of Science and Technology. Medical sciences |
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Publisher | Springer Berlin Heidelberg The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China |
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References | Park, Liu, Hu (CR1) 2008; 322 Zhu, Hoell, Ahlemeyer (CR8) 2007; 50 Kurimoto, Yin, Omura (CR7) 2010; 30 Walker, Walker, Liu (CR3) 2012; 7 Liu, Lu, Lee (CR6) 2010; 13 Abe, Borson, Gambello (CR5) 2010; 285 Yang (CR4) 2010; 41 Wu, Wang, Cai (CR9) 2010; 16 Christie, Webber, Martinez (CR2) 2010; 30 17169462 - Neurochem Int. 2007 Feb;50(3):507-16 20610765 - J Neurosci. 2010 Jul 7;30(27):9306-15 20694004 - Nat Neurosci. 2010 Sep;13(9):1075-81 18988856 - Science. 2008 Nov 7;322(5903):963-6 21084621 - J Neurosci. 2010 Nov 17;30(46):15654-63 20615870 - J Biol Chem. 2010 Sep 3;285(36):28034-43 22253859 - PLoS One. 2012;7(1):e30012 HR Wu (1234_CR9) 2010; 16 P Yang (1234_CR4) 2010; 41 T Kurimoto (1234_CR7) 2010; 30 Y Zhu (1234_CR8) 2007; 50 KK Park (1234_CR1) 2008; 322 N Abe (1234_CR5) 2010; 285 K Liu (1234_CR6) 2010; 13 KJ Christie (1234_CR2) 2010; 30 CL Walker (1234_CR3) 2012; 7 |
References_xml | – volume: 50 start-page: 507 issue: 3 year: 2007 end-page: 516 ident: CR8 article-title: Implication of PTEN in production of reactive oxygen species and neuronal death in in vitro models of stroke and Parkinson’s disease publication-title: Neurochem Int doi: 10.1016/j.neuint.2006.10.010 contributor: fullname: Ahlemeyer – volume: 16 start-page: 1607 issue: 11 year: 2010 end-page: 1610 ident: CR9 article-title: Roles of PTEN in neuronal injury and brain disorders publication-title: Yixue Zongshu (Chinese) contributor: fullname: Cai – volume: 30 start-page: 9306 issue: 27 year: 2010 end-page: 9315 ident: CR2 article-title: PTEN inhibition to facilitate intrinsic regenerative outgrowth of adult peripheral axons publication-title: J Neurosci doi: 10.1523/JNEUROSCI.6271-09.2010 contributor: fullname: Martinez – volume: 7 start-page: e30012 issue: 1 year: 2012 ident: CR3 article-title: Systemic bisperoxovanadium activates Akt/mTOR, reduces autophagy, and enhances recovery following cervical spinal cord injury publication-title: PLoS One doi: 10.1371/journal.pone.0030012 contributor: fullname: Liu – volume: 285 start-page: 28 034 issue: 36 year: 2010 end-page: 28 043 ident: CR5 article-title: Mammalian target of rapamycin (mTOR) activation increases axonal growth capacity of injured peripheral nerves publication-title: J Biol Chem doi: 10.1074/jbc.M110.125336 contributor: fullname: Gambello – volume: 30 start-page: 15654 issue: 46 year: 2010 end-page: 15663 ident: CR7 article-title: Long-distance axon regeneration in the mature optic nerve: contributions of oncomodulin, cAMP, and pten gene deletion publication-title: J Neurosci doi: 10.1523/JNEUROSCI.4340-10.2010 contributor: fullname: Omura – volume: 41 start-page: 313 issue: 4 year: 2010 end-page: 316s ident: CR4 article-title: Promoting the repair of CNS by targeting PTEN publication-title: Prog Phys Sci (Chinese) contributor: fullname: Yang – volume: 13 start-page: 1075 issue: 9 year: 2010 end-page: 1081 ident: CR6 article-title: PTEN deletion enhances the regenerative ability of adult corticospinal neurons publication-title: Nat Neurosci doi: 10.1038/nn.2603 contributor: fullname: Lee – volume: 322 start-page: 963 issue: 5903 year: 2008 end-page: 966 ident: CR1 article-title: Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway publication-title: Science doi: 10.1126/science.1161566 contributor: fullname: Hu – volume: 30 start-page: 9306 issue: 27 year: 2010 ident: 1234_CR2 publication-title: J Neurosci doi: 10.1523/JNEUROSCI.6271-09.2010 contributor: fullname: KJ Christie – volume: 16 start-page: 1607 issue: 11 year: 2010 ident: 1234_CR9 publication-title: Yixue Zongshu (Chinese) contributor: fullname: HR Wu – volume: 7 start-page: e30012 issue: 1 year: 2012 ident: 1234_CR3 publication-title: PLoS One doi: 10.1371/journal.pone.0030012 contributor: fullname: CL Walker – volume: 41 start-page: 313 issue: 4 year: 2010 ident: 1234_CR4 publication-title: Prog Phys Sci (Chinese) contributor: fullname: P Yang – volume: 50 start-page: 507 issue: 3 year: 2007 ident: 1234_CR8 publication-title: Neurochem Int doi: 10.1016/j.neuint.2006.10.010 contributor: fullname: Y Zhu – volume: 322 start-page: 963 issue: 5903 year: 2008 ident: 1234_CR1 publication-title: Science doi: 10.1126/science.1161566 contributor: fullname: KK Park – volume: 285 start-page: 28 034 issue: 36 year: 2010 ident: 1234_CR5 publication-title: J Biol Chem doi: 10.1074/jbc.M110.125336 contributor: fullname: N Abe – volume: 13 start-page: 1075 issue: 9 year: 2010 ident: 1234_CR6 publication-title: Nat Neurosci doi: 10.1038/nn.2603 contributor: fullname: K Liu – volume: 30 start-page: 15654 issue: 46 year: 2010 ident: 1234_CR7 publication-title: J Neurosci doi: 10.1523/JNEUROSCI.4340-10.2010 contributor: fullname: T Kurimoto |
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In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and... In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote... |
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SubjectTerms | Animals Axons - drug effects Axons - physiology Cells, Cultured Delayed-Action Preparations - chemistry Delayed-Action Preparations - pharmacokinetics Delayed-Action Preparations - pharmacology Drug Compounding Female Ganglia, Spinal - drug effects Ganglia, Spinal - metabolism Ganglia, Spinal - physiology Immunohistochemistry Lactic Acid - chemistry Lactic Acid - pharmacokinetics Lactic Acid - pharmacology Medicine Medicine & Public Health Microscopy, Electron Microspheres Neural Stem Cells - drug effects Neural Stem Cells - physiology Neurofilament Proteins - metabolism Neurons - drug effects Neurons - metabolism Organometallic Compounds - chemistry Organometallic Compounds - pharmacokinetics Organometallic Compounds - pharmacology Polyesters Polymers - chemistry Polymers - pharmacokinetics Polymers - pharmacology Pregnancy Rats |
Title | Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells |
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