Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells

Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dor...

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Published inJournal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 1; pp. 76 - 80
Main Authors Lin, Qiang, Chen, Hai-yun, Li, Hao-shen, Cai, Yang-ting
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014
The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China
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Abstract Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
AbstractList In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
Author Lin, Qiang
Cai, Yang-ting
Li, Hao-shen
Chen, Hai-yun
AuthorAffiliation The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China
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  surname: Cai
  fullname: Cai, Yang-ting
  organization: The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine
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Cites_doi 10.1016/j.neuint.2006.10.010
10.1523/JNEUROSCI.6271-09.2010
10.1371/journal.pone.0030012
10.1074/jbc.M110.125336
10.1523/JNEUROSCI.4340-10.2010
10.1038/nn.2603
10.1126/science.1161566
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Issue 1
Keywords controlled release
microspheres
neural stem cells
PLLA/bpV(pic)
dorsal root ganglion
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The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China
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Snippet Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and...
In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote...
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SubjectTerms Animals
Axons - drug effects
Axons - physiology
Cells, Cultured
Delayed-Action Preparations - chemistry
Delayed-Action Preparations - pharmacokinetics
Delayed-Action Preparations - pharmacology
Drug Compounding
Female
Ganglia, Spinal - drug effects
Ganglia, Spinal - metabolism
Ganglia, Spinal - physiology
Immunohistochemistry
Lactic Acid - chemistry
Lactic Acid - pharmacokinetics
Lactic Acid - pharmacology
Medicine
Medicine & Public Health
Microscopy, Electron
Microspheres
Neural Stem Cells - drug effects
Neural Stem Cells - physiology
Neurofilament Proteins - metabolism
Neurons - drug effects
Neurons - metabolism
Organometallic Compounds - chemistry
Organometallic Compounds - pharmacokinetics
Organometallic Compounds - pharmacology
Polyesters
Polymers - chemistry
Polymers - pharmacokinetics
Polymers - pharmacology
Pregnancy
Rats
Title Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells
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