Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 1; pp. 76 - 80
• Main Authors: Lin, Qiang, Chen, Hai-yun, Li, Hao-shen, Cai, Yang-ting
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014; The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China
• Subjects: Animals; Axons - drug effects; Axons - physiology; Cells, Cultured; Delayed-Action Preparations - chemistry; Delayed-Action Preparations - pharmacokinetics; Delayed-Action Preparations - pharmacology; Drug Compounding; Female; Ganglia, Spinal - drug effects; Ganglia, Spinal - metabolism; Ganglia, Spinal - physiology; Immunohistochemistry; Lactic Acid - chemistry; Lactic Acid - pharmacokinetics; Lactic Acid - pharmacology; Medicine; Medicine & Public Health; Microscopy, Electron; Microspheres; Neural Stem Cells - drug effects; Neural Stem Cells - physiology; Neurofilament Proteins - metabolism; Neurons - drug effects; Neurons - metabolism; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacokinetics; Organometallic Compounds - pharmacology; Polyesters; Polymers - chemistry; Polymers - pharmacokinetics; Polymers - pharmacology; Pregnancy; Rats; controlled release; microspheres; neural stem cells; PLLA/bpV(pic); dorsal root ganglion
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1234-z

Summary In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.