Expression of heparanase mRNA in anti-sense oligonucleotide - transfected human esophageal cancer EC9706 cells

• Published in: World journal of gastroenterology : WJG Vol. 11; no. 31; pp. 4916 - 4917
• Main Authors: Chen, Kui-Sheng, Zhang, Lan, Tang, Lin, Zhang, Yun-Han, Gao, Dong-Ling, Yan, Liang, Zhang, Lei
• Format: Journal Article
• Language: English
• Published: United States Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China 21.08.2005; Baishideng Publishing Group Inc
• Subjects: Brief Reports; Cell Line, Tumor; EC9706; Esophageal Neoplasms; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Glucuronidase - genetics; Humans; mRNA; Oligonucleotides, Antisense - genetics; RNA, Messenger - genetics; Transfection; 基因表达; 寡核苷酸; 肝疾病; 食管癌; Heparanase; Esophageal cancer EC9706 cells; In situ hybridization; Anti-sense oligonucleotides
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i31.4916

AIM： To investigate the effed3 of anti-sense oligonucleotides （ASODNs） on mRNA expression of heparanase in human esophageal cancer EC9706 cells. METHODS： One non-sense oligonucleotide （N-ODN） and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization. RESULTS： The expression of heparanase mRNA could be inhibited by ASODNs.There was no significant difference among five ASODNs （P〉0.05）, but there was a significant difference between ASODNs and N-ODN or non-transfected group （ASODNI： 2.25±0.25, ASODN2： 2.21±0.23, ASODN3： 2.23±0.23, ASODN4：2.25±0.24 vs N-ODN： 3.47±2.80 or non- transfected group： 3.51±2.93 respectively, P〈0.05）. CONCLUSION： The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.