Traditional Pediatric Massage Enhanced Hippocampal GR, BDNF and IGF-1 Expressions and Exerted an Anti-depressant Effect in an Adolescent Rat Model of CUMS-induced Depression

• Published in: Neuroscience Vol. 542; pp. 47 - 58
• Main Authors: Wu, Rong, Xiong, Ying, Gu, Yun, Cao, Li-Yue, Zhang, Shu-Ying, Song, Zhi-Xiu, Fan, Pu, Lin, Lin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.03.2024
• Subjects: adolescence; Adolescent; Animals; Antidepressive Agents - metabolism; Brain-Derived Neurotrophic Factor - metabolism; Child; depression; Depression - metabolism; Disease Models, Animal; glucocorticoid receptor; Hippocampus - metabolism; Humans; Insulin-Like Growth Factor I - metabolism; insulin-like growth factor-1; Massage; Rats; Receptors, Glucocorticoid - metabolism; Stress, Psychological - metabolism; Sucrose - metabolism; Weight Gain; glucocorticoid receptor; MWM; EPM; BDNF; CUMS; insulin-like growth factor-1; IGF-1; GR; TPM; CORT; FLX; SPT; adolescence; HPA; depression; massage; GC; BSM
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2024.01.025

•CUMS induced depression-related behaviors in adolescent rats.•CUMS decreased the hippocampal expressions of IGF-1, GR and BDNF.•Traditional pediatric massage (TPM) prevented depression in adolescent rats.•TPM enhanced the hippocampal expressions of IGF-1, GR and BDNF.•TPM had a better anti-depressant effect than back stroking and fluoxetine. This study aimed to investigate the anti-depressant effect of traditional pediatric massage (TPM) in adolescent rats and its possible mechanism. The adolescent depression model in rats was established by using chronic unpredictable mild stress (CUMS). All rats were randomly divided into five groups (seven per group), including the groups of control (CON), CUMS, CUMS with TPM, CUMS with back stroking massage (BSM) and CUMS with fluoxetine (FLX). The tests of sucrose preference, Morris water maze and elevated plus maze were used to evaluate depression-related behaviors. Plasma corticosterone (CORT) level was measured by ELISA. The gene and protein expressions of glucocorticoid receptor (GR), brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-1 (IGF-1) were measured by RT-qPCR and IHC respectively. The results showed that CUMS induced depression-related behaviors in the adolescent rats, along with decreased weight gain and reduced hippocampal expressions of GR, IGF-1 and BDNF. TPM could effectively prevent depression-related behaviors in CUMS-exposed adolescent rats, manifested as increasing weight gain, sucrose consumption, ratio of open-arm entry, times of crossing the specific quadrant and shortening escape latency. TPM also decreased CORT level in plasma, together with enhancing expressions of GR, IGF-1 and BDNF in the hippocampus. These results may support the clinical application of TPM to prevent and treat adolescent depression.