Mass Spectral Characterization and UPLC Quantitation of 3-Deoxyanthocyanidins in Sorghum bicolor Varietals

A quantitative ultra-performance LC (UPLC) method was developed and validated to successfully separate, identify, and quantitate the major polyphenolic compounds present in different varieties of sorghum (Sorghum bicolor) feedstock. The method was linear from 3.2 to 320 ppm, with an r2 of 0.99999 wh...

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Bibliographic Details
Published inJournal of AOAC International Vol. 101; no. 1; pp. 242 - 248
Main Authors Stern, Nathan P, Rana, Jatinder, Chandra, Amitabh, Balles, John
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.01.2018
Subjects
Anthocyanins - analysis
Chemical properties
Chromatography, High Pressure Liquid
Mass Spectrometry
Measurement
Plant pigments
Sorghum
Sorghum - chemistry
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Summary:A quantitative ultra-performance LC (UPLC) method was developed and validated to successfully separate, identify, and quantitate the major polyphenolic compounds present in different varieties of sorghum (Sorghum bicolor) feedstock. The method was linear from 3.2 to 320 ppm, with an r2 of 0.99999 when using luteolinidin chloride as the external standard. Method accuracy was determined to be 99.5%, and precision of replicate preparations was less than 1% RSD. Characterization by UPLC-MS determined that the predominant polyphenolic components of the sorghum varietals were 3-deoxyanthocyanidins (3-DXAs). High-throughput screening for 3-DXA identified four unique classes within the sorghum varieties. Certain feedstock varieties have been found to have a high potential to not only be plant-based colorants, but also provide significant amounts of bioactive 3-DXAs, making them of unique interest to the dietary supplement industry.
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ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.16-0338