Relationship between Renalase Expression and Kidney Disease: an Observational Study in 72 Patients Undergoing Renal Biopsy

• Published in: Current medical science Vol. 38; no. 2; pp. 268 - 276
• Main Authors: Huang, Yi-sha, Lai, Jian-bo, Li, Sheng-fa, Wang, Ting, Liu, Ying-nan, Zhang, Qing-xia, Zhang, Shu-yuan, Sun, Chun-han, Hu, Nan, Zhang, Xin-zhou
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.04.2018; Key Renal Laboratory of Shenzhen, Shenzhen 518108, China%Department of Gastrointestinal, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518000, China%Department of Orthopedics, Huizhou First Hospital, Affiliated Hospital of Guangdong Medical University, Huizhou 516000, China%Department of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China%Department of Hand Microsurgry, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518000, China; Department of Nephrology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518000, China
• Subjects: Medicine; Medicine & Public Health; kidney disease; renal function; renal puncture biopsy; renalase; flavoprotein
• ISSN: 2096-5230; 2523-899X; 1672-0733; 2523-899X
• DOI: 10.1007/s11596-018-1875-4

Summary The relationship between the levels of renalase and changes in proteinuria, hypertension, renal function, renal tubular epithelial cell apoptosis and B-cell lymphoma-2 (Bcl-2) expression was investigated in patients (chronic nephritis, primary nephrotic syndrome or other kidney disease) that underwent renal biopsy. The study group comprised 72 patients undergoing renal biopsy. Patient profiles and renal function were collected. Concentrations of renalase and Bcl-2 were measured by immunohistochemistry. Tubular injury was detected by periodic acid Schiff staining (PAS) and renal tubular epithelial cell apoptosis was assessed by TUNEL assay. The expression of renalase was significantly lower in renal biopsy specimens than in normal kidney tissues. There was a positive linear relationship between renalase and some serum and cardiac indices; a negative correlation was found between age, eGFR, Ccr and 24-h urinary protein. Renal tubule injury index and tubular epithelial cell apoptosis index showed a negative linear correlation with renalase. The results showed that renalase probably increased the expression of Bcl-2. By two independent samples t -test, renalase levels were significantly increased in the non-hypertension group than in the hypertension group. One-way ANOVA showed that renalase expression was higher in samples with Lee’s grade III than in those with Lee’s grade V. The expression of renalase was significantly decreased in patients who underwent renal biopsy, and was also associated with blood and renal function. The research proved that renalase may reduce renal tubular injury and apoptosis of renal tubular epithelial cells through the mitochondrial apoptosis pathway, finally achieving the purpose of delaying the progress of renal failure.