Ontogenetic and temperature-dependent changes in tolerance to hypoxia and hydrogen sulfide during the early life stages of the Manila clam Ruditapes philippinarum

• Published in: Marine environmental research Vol. 137; pp. 177 - 187
• Main Authors: Kodama, Keita, Waku, Mitsuyasu, Sone, Ryota, Miyawaki, Dai, Ishida, Toshiro, Akatsuka, Tetsuji, Horiguchi, Toshihiro
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2018; Elsevier BV
• Subjects: Adaptation, Physiological; Animals; Anoxia; Aquatic organisms; Benthos; Biological fertilization; Bivalve; Bivalvia - physiology; Body size; Coastal ecosystem; Coastal zone; Early life history; Ecosystem biology; Eutrophication; Exposure; Fertilization; Hydrogen sulfide; Hydrogen Sulfide - toxicity; Hydrogen sulphide; Hypoxia; Juveniles; Larva; Larvae; Marine molluscs; Ocean circulation; Ontogeny; Ruditapes philippinarum; Sulphides; Temperature; Temperature dependence; Temperature tolerance; Upwelling; Water temperature; Wind effects; Hydrogen sulfide; Anoxia; Coastal ecosystem; Body size; Hypoxia; Benthos; Early life history; Bivalve; Water temperature; Eutrophication
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/j.marenvres.2017.12.019

Wind-induced upwelling of hypoxic waters containing hydrogen sulfide (H2S) sometimes causes mass mortalities of aquatic organisms inhabiting coastal areas, including the hypoxia-tolerant Manila clam Ruditapes philippinarum. We examined the tolerance of Manila clam to H2S under controlled laboratory conditions. Larvae and juveniles obtained by artificial fertilization or from a wild population were exposed to normoxic or to hypoxic water with or without un-ionized H2S (concentrations, 0.2–52.2 mg/L). Twenty-four-hour exposure experiments revealed ontogenetic changes in the clam's tolerance to H2S exposure: tolerance was enhanced from the larval stages to juveniles just after settlement but was attenuated as juveniles grew. Tolerance of larvae and juveniles to H2S exposure weakened as the water temperature rose from 20 to 28 °C. Prolonged 48-h exposure to H2S attenuated the tolerance of juveniles to H2S. Temporary suspension of H2S exposure by 24-h reoxygenation improved the ability of juveniles to withstand repeated H2S exposure. •We examined the tolerance of Manila clam larvae and juveniles to H2S exposure.•H2S tolerance was enhanced from larval stages to juveniles just after settlement.•Tolerance to H2S was attenuated as juveniles grew.•H2S tolerance of larvae and juveniles weakened as the water temperature rose.•Ability to withstand repeated H2S exposure improved with temporary reoxygenation.