DNA microarray for identification of the herb of Dendrobium species from Chinese medicinal formulations

A DNA microarray for detecting processed medicinal Dendrobium species (Herba Dendrobii) was constructed by incorporating the ITS1-5.8S-ITS2 sequences of 16 Dendrobium species on a glass slide. Using fluorescence-labeled ITS2 sequences as probes, distinctive signals were obtained for the five medicin...

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Bibliographic Details
Published inPlanta medica Vol. 69; no. 12; pp. 1172 - 1174
Main Authors Zhang, Y.B, Wang, J, Wang, Z.T, But, P.P.H, Shaw, P.C
Format Journal Article
LanguageEnglish
Published Germany 01.12.2003
Subjects
Dendrobium
Dendrobium - genetics
Dendrobium nobile
DNA, Plant - analysis
Drugs, Chinese Herbal - analysis
herbaceous plants
Humans
internal transcribed spacers
microarray technology
Oligonucleotide Array Sequence Analysis
Phytotherapy
plant genetics
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Summary:A DNA microarray for detecting processed medicinal Dendrobium species (Herba Dendrobii) was constructed by incorporating the ITS1-5.8S-ITS2 sequences of 16 Dendrobium species on a glass slide. Using fluorescence-labeled ITS2 sequences as probes, distinctive signals were obtained for the five medicinal Dendrobium species listed in the Chinese Pharmacopoeia. The established microarray was able to detect the presence of D. nobile in a Chinese medicinal formulation containing nine herbal components.
ISSN:0032-0943
1439-0221
DOI:10.1055/s-2003-818015