Association between eNOS polymorphisms and risk of coronary artery disease in a Korean population: a meta-analysis

• Published in: Genetics and molecular research Vol. 14; no. 4; pp. 16508 - 16520
• Main Authors: Sung, J H, Lee, B E, Kim, J O, Jeon, Y J, Kim, S H, Lim, S W, Moon, J Y, Cha, D H, Kim, O J, Kim, I J, Kim, N K
• Format: Journal Article
• Language: English
• Published: Brazil 09.12.2015
• Subjects: Aged; Alleles; Asian Continental Ancestry Group - genetics; Case-Control Studies; Comorbidity; Coronary Artery Disease - blood; Coronary Artery Disease - epidemiology; Coronary Artery Disease - genetics; Female; Folic Acid - blood; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Haplotypes; Homocysteine - blood; Humans; Linkage Disequilibrium; Male; Middle Aged; Nitric Oxide Synthase Type III - genetics; Odds Ratio; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Republic of Korea - epidemiology; Risk; Risk Factors; Republic of Korea
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.December.9.23

Coronary artery disease (CAD), a multifactorial disease, is a common cause of mortality in humans. Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (-786T>C, 4a4b, and 894G>T) have been previously associated with increased CAD risk. However, the sample size of this previous study was too small and limited to comprehensively define an association between eNOS polymorphisms and CAD; therefore, this analysis was duplicated with a larger population. The study was conducted on 559 patients with CAD and 574 healthy controls. Genetic DNA was extracted using the commercial G-DEX blood extraction kit and statistical analyses were performed on the GraphPad prism 4.0 and MedCalc 12.0 statistical software platforms. No single variant of the eNOS polymorphism was associated with CAD risk. The combination genotypes of eNOS -786TT/4a4b+4a4a [adjusted odds ratio (AOR) = 0.122; 95% confidence interval (CI): 0.042-0.358] and eNOS -786TC+CC/4b4b (AOR = 0.379; 95%CI: 0.147-0.979) were associated with decreased CAD incidence. Haplotype analysis revealed that the T-4a haplotype of eNOS -786T>C and 4a4b exerted a protective effect against CAD. The association between eNOS -786T>C and increased CAD risk was not replicated in this (larger) population. However, some combined genotypes showed a meaningful association with CAD risk.