Mercury chloride effects on the function and cellular integrity of sea bass ( Dicentrarchus labrax) head kidney macrophages

The objective of this work was to study mercury chloride effects on the function and integrity of sea bass ( Dicentrarchus labrax) head kidney macrophages (S-HKM), and to evaluate the response of HgCl 2-exposed cells to macrophage activating factor(s) (MAF) produced by sea bass head kidney leukocyte...

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Bibliographic Details
Published inFish & shellfish immunology Vol. 17; no. 5; pp. 489 - 498
Main Authors Sarmento, Amélia, Guilhermino, Lúcia, Afonso, António
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.11.2004
Subjects
Animals
Apoptosis
Apoptosis - drug effects
Apoptosis - immunology
Aquaculture
Bass - immunology
Dicentrarchus labrax
Flow Cytometry
Kidney - immunology
Macrophage Activation - drug effects
Macrophage Activation - immunology
Macrophage-Activating Factors - biosynthesis
Macrophage-Activating Factors - immunology
Macrophages
Mercuric Chloride - toxicity
Mercury chloride
Microscopy, Fluorescence
Phagocytosis
Phagocytosis - drug effects
Phagocytosis - immunology
Photobacterium - immunology
Reactive Oxygen Species - immunology
ROS
Sea bass
Mercury chloride
Macrophages
Sea bass
Phagocytosis
ROS
Apoptosis
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Summary:The objective of this work was to study mercury chloride effects on the function and integrity of sea bass ( Dicentrarchus labrax) head kidney macrophages (S-HKM), and to evaluate the response of HgCl 2-exposed cells to macrophage activating factor(s) (MAF) produced by sea bass head kidney leukocytes. There was considerable variability in the effects of HgCl 2 on the production of reactive oxygen species (ROS) by S-HKM. When incubated with HgCl 2, cells from five out of nine fish tested showed a decrease in ROS production as compared to cells incubated with medium alone. In those cultures, MAF addition prevented the mercury chloride-induced decrease in ROS production. In other S-HKM cultures isolated from different fish, mercury chloride abrogated the up-regulating effect of MAF on the respiratory burst. MAF activation of the phagocytic activity of S-HKM was also impaired by HgCl 2 addition. Mercury chloride induced apoptosis in S-HKM cultures and MAF addition prevented this effect.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2004.05.004