Cecropin P1 antimicrobial peptide modulates differential expression of immune relevant genes in rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1

• Published in: Fish & shellfish immunology Vol. 137; no. C; p. 108756
• Main Authors: Han, Yueh-Chiang, Shepherd, Brian S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2023; Elsevier
• Subjects: Animals; Antimicrobial peptide; Antimicrobial Peptides; Cecropin P1; Cell Line; Gills; Immunomodulation; Inflammation; Lipopolysaccharides - pharmacology; Mucosal immunity; Oncorhynchus mykiss - genetics; Peptides - genetics; Rainbow trout; RTgill-W1 cell; Mucosal immunity; RTgill-W1 cell; Immunomodulation; Cecropin P1; Rainbow trout; Antimicrobial peptide
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2023.108756

Accumulated evidence indicates that antimicrobial peptides modulate immune activities in fish. In this study, we profiled the differential expression patterns of representative immune relevant genes in an epithelial-like cell line of rainbow trout gill, RTgill-W1, in response to exposure of cecropin P1 antimicrobial peptide. RTgill-W1 cells were treated with synthetic cecropin P1 over time (0, 2, 4 and 24 h) with or without the present of lipopolysaccharide (LPS) or polyinosinic polycytidylic acid (PolyI:C). The relative abundances of each mRNA were measured by real-time quantitative PCR. The dose-response study revealed significant perturbations of mRNA levels of genes related to pro-inflammation, acute phase, surface proteins and transcription factors at 30 μM of cecropin P1. In addition, cecropin P1 altered the differential expression patterns that were induced by LPS or PolyI:C, at different time points in RTgill-W1. Overall, our results indicate that cecropin P1 exhibits pro-inflammation activity, modulate cell-cell interaction and cytokine signal transduction in rainbow trout gill cell, and may suggest a potential application of this peptide as an immune adjuvant for disease control in aquaculture. •Utilizing RTgill-W1 cell line to build an in vitro model to represent the function of gill organ, studying differential expression profiles of immune relevant genes.•Cecropin P1 exerts pro-inflammatory effects and enhances inductions from LPS and PolyI:C treatments on RTgill-W1 cells.•Results provide better understandings of gill mucosal immunity, and may lead to development of novel applications that improve disease control in aquaculture production of rainbow trout.