MiR-25 regulates cell proliferation and metastasis in bladder urothelial carcinoma

Background: Bladder urothelial carcinoma (BC) is a common malignant tumor with a high incidence. This study aims to explore the role of miR-25 in BC tumorigenesis. Material and Methods: The expression of miR-25 and PTEN were detected in clinical BC tissues. BC cell lines T24 and 5637 were used to tr...

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Bibliographic Details
Published inJournal of Cancer Vol. 12; no. 22; pp. 6706 - 6714
Main Authors Ning, Jin-zhuo, Chu, Chuan-min, Du, Yang, Zuo, Li
Format Journal Article
LanguageEnglish
Published Wyoming Ivyspring International Publisher Pty Ltd 01.01.2021
Ivyspring International Publisher
Subjects
Antibodies
Apoptosis
Binding sites
Biomarkers
Bladder cancer
Cell growth
Medical prognosis
Metastasis
MicroRNAs
Research Paper
Statistical analysis
United States > US
China
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Summary:Background: Bladder urothelial carcinoma (BC) is a common malignant tumor with a high incidence. This study aims to explore the role of miR-25 in BC tumorigenesis. Material and Methods: The expression of miR-25 and PTEN were detected in clinical BC tissues. BC cell lines T24 and 5637 were used to transfect miR-25 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-25 and PTEN. CCK-8 method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasion ability were examined by transwell assays. Western blotting detects the protein levels of PTEN, β-catenin, GSK-3β and p-GSK-3β. Results: MiR-25 and PTEN expression are found to be negatively correlated in BC tissues. Further research confirmed that PTEN is a direct target of miR-25. In addition, the overexpression of miR-25 down-regulates the expression of PTEN, induces cell survival and inhibits apoptosis, while the knockout of miR-25 leads to the opposite result. miR-25 also inhibits the phosphorylation of GSK-3β and β-catenin without changing the total level of GSK-3β. In vivo experiments confirmed that miR-25 plays an oncogene's role by regulating the PTEN and Wnt/β-catenin signaling pathways. Conclusion: Our research shows that miR-25 has a negative regulatory effect on the expression of PTEN in clinical specimens and in vitro. miR-25 can promote the proliferation of BC cells and induce cell invasion. Therefore, miR-25 may be used as a biomarker to predict the progression of BC.
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These authors contributed equally to this work.
Competing Interests: The authors have declared that no competing interest exists.
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.62743