Targeting of monoclonal antibody-coated liposomes to sheep red blood cells

• Published in: Biochemical and biophysical research communications Vol. 103; no. 3; pp. 1069 - 1076
• Main Authors: Harsch, M., Walther, P., Weder, H.G., Hengartner, H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.12.1981
• Subjects: Animals; Antibodies, Monoclonal - administration & dosage; Erythrocytes; Horses; In Vitro Techniques; Liposomes - administration & dosage; Mice; Microscopy, Electron, Scanning; Sheep
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(81)90917-7

A monoclonal mouse anti-sheep red blood cell specific antibody IgG 2b was esterified with palmitic acid which served as a hydrophobic anchor for successfull incorporation into the liposomal membrane. The formation of coated liposomes by dialyzing the mixed antibody/lipid/detergent micelles against phosphate buffer was simplified b by using the same detergent as for the antibody derivatization. No purification step of any intermediate product was necessary. Targeting of the resulting vesicles to sheep red blood cells occured with high efficiency compared with control liposomes. The uptake was fast and specific as demonstrated with sheep and horse red blood cells by the use of radioactively labelled liposomes and by scanning electron microscopy.