Polyphenols from Maackia amurensis Heartwood Protect Neuronal Cells from Oxidative Stress and Prevent Herpetic Infection

• Published in: International journal of molecular sciences Vol. 25; no. 8; p. 4142
• Main Authors: Tarbeeva, Darya V, Pislyagin, Evgeny A, Menchinskaya, Ekaterina S, Berdyshev, Dmitrii V, Krylova, Natalya V, Iunikhina, Olga V, Kalinovskiy, Anatoliy I, Shchelkanov, Mikhail Y, Mishchenko, Natalia P, Aminin, Dmitry L, Fedoreyev, Sergey A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.04.2024
• Subjects: Animals; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Cell Survival - drug effects; Hepatitis; Herpes Simplex - drug therapy; Herpes viruses; Herpesvirus 1, Human - drug effects; HSV-1; Humans; Infections; Membrane Potential, Mitochondrial - drug effects; Mice; Neurons - drug effects; Neurons - metabolism; neuroprotective activity; Neuroprotective Agents - chemistry; Neuroprotective Agents - pharmacology; Oxidative stress; Oxidative Stress - drug effects; Pathogenesis; Plant Extracts - chemistry; Plant Extracts - pharmacology; polyphenolic compounds; Polyphenols; Polyphenols - chemistry; Polyphenols - pharmacology; Reactive Oxygen Species - metabolism; ROS; Severe acute respiratory syndrome coronavirus 2; virucidal activity; Russia; HSV-1; neuroprotective activity; ROS; polyphenolic compounds; virucidal activity
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25084142

Here, we continued the investigation of anti-HSV-1 activity and neuroprotective potential of 14 polyphenolic compounds isolated from heartwood. We determined the absolute configurations of asymmetric centers in scirpusin A ( ) and maackiazin ( ) as 7 ,8 and 1″ ,2″ , respectively. We showed that dimeric stilbens maackin ( ) and scirpusin A ( ) possessed the highest anti-HSV-1 activity among polyphenols - . We also studied the effect of polyphenols and on the early stages of HSV-1 infection. Direct interaction with the virus (virucidal activity) was the main mechanism of the antiviral activity of these compounds. The neuroprotective potential of polyphenolic compounds from was studied using models of 6-hydroxydopamine (6-OHDA)-and paraquat (PQ)-induced neurotoxicity. A dimeric stilbene scirpusin A ( ) and a flavonoid liquiritigenin ( ) were shown to be the most active compounds among the tested polyphenols. These compounds significantly increased the viability of 6-OHDA-and PQ-treated Neuro-2a cells, elevated mitochondrial membrane potential and reduced the intracellular ROS level. We also found that scirpusin A ( ), liquiritigenin ( ) and retusin ( ) considerably increased the percentage of live Neuro-2a cells and decreased the number of early apoptotic cells. Scirpusin A ( ) was the most promising compound possessing both anti-HSV-1 activity and neuroprotective potential.