Fasciola hepatica: A proteolytic digestive enzyme

A proteolytic enzyme in the gut exudate of the common liver fluke has been purified. The enzyme is specific for globin with a pH optimum of 3.9–4.0 and breaks the protein down to peptides and a small percentage of free amino acids. Collagenase activity at pH 7.5 copurifies with the main globinolytic...

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Bibliographic Details
Published inExperimental parasitology Vol. 49; no. 2; pp. 281 - 287
Main Authors Simpkin, K.G., Chapman, C.R., Coles, G.C.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1980
Subjects
Amino acid composition
Amino Acids - analysis
Animals
Antigenicity
Antigens
Digestion products
Fasciola hepatica
Fasciola hepatica - enzymology
Fluke, liver
Globins - metabolism
Hemoglobins - metabolism
Kinetics
Michaelis constant
Molecular Weight
Peptide Hydrolases - analysis
Peptide Hydrolases - isolation & purification
Peptide Hydrolases - metabolism
Proteolytic enzyme
Substrate Specificity
Trematode, digenetic
Fasciola hepatica
Trematode, digenetic
Antigenicity
Michaelis constant
Proteolytic enzyme
Fluke, liver
Amino acid composition
Digestion products
Molecular weight
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Summary:A proteolytic enzyme in the gut exudate of the common liver fluke has been purified. The enzyme is specific for globin with a pH optimum of 3.9–4.0 and breaks the protein down to peptides and a small percentage of free amino acids. Collagenase activity at pH 7.5 copurifies with the main globinolytic enzyme. The enzyme has a molecular weight of 12,000 and does not appear to be antigenic in fluke-infected animals.
Bibliography:L
L72
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ISSN:0014-4894
1090-2449
DOI:10.1016/0014-4894(80)90125-3