Recovery of potassium clavulanate from fermentation broth by ion exchange chromatography and desalting electrodialysis

• Published in: Biotechnology and bioprocess engineering Vol. 14; no. 6; pp. 803 - 810
• Main Authors: Kim, Hyun Han, Kang, Sang Hyeon, Chang, Yong Keun
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.12.2009; Springer Nature B.V; 한국생물공학회
• Subjects: Antibiotics; Bioengineering; Biotechnology; By products; Chemical reactions; Chemistry; Chemistry and Materials Science; Chromatography; Electrodialysis; Equilibrium; Experiments; Feed composition; Fermentation; Flow rates; Hemodialysis; Industrial and Production Engineering; Ion exchange; Potassium; Solvent extraction processes; Studies; 생물공학; ion exchange chromatography (IEC); desaiting electrodialysis (DSED); potassium clavulanate (KCA); fermentation broth
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-008-0161-x

Ion exchange chromatography (IEC) and desalting electrodialysis (DSED) processes were developed for the recovery and purification of potassium clavulanate (KCA) from fermentation broth. A strong anion exchanger, Amberlite IRA 400 resin, a potassium acetate solution as equilibrium buffer, and a potassium chloride (KCl) solution as elution buffer were used for the recovery of KCA in IEC. In order to determine optimal operating conditions, the effects of various operating parameters such as equilibrium buffer pH and concentration, elution buffer concentration, gradient length, and volumetric flow rate on KCA recovery and by-product removal were investigated using a simulated fermentation broth. In the subsequent step of DSED, employing cation (Neocepta CMS, Tokuyama, Japan) and anion (Neocepta ACS, Tokuyama, Japan) exchange membranes were carried out to remove KCl that existed in a large amount in the ion exchanged solution. The effects of operation voltage and feed composition on the performance of DSED were investigated. Based on the operating conditions determined above, IEC and DSED were applied in sequence to an ultrafiltered fermentation broth. Almost complete removal of KCl was possible with no significant loss of KCA, although the KCA recovery was slightly lower than that with the simulated fermentation broth. Based on this observation, it was concluded that IEC and DESD could be an effective process combination for the recovery of KCA from fermentation broth.