Allotetraploidy of Zoysia species with 2n = 40 based on a RFLP genetic map

• Published in: Theoretical and applied genetics Vol. 98; no. 5; pp. 751 - 756
• Main Authors: Yaneshita, M, Kaneko, S, Sasakuma, T
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.04.1999; Berlin Springer Nature B.V
• Subjects: Biological and medical sciences; Chromosome mapping; Chromosomes; Classical genetics, quantitative genetics, hybrids; Cloning; Enzymes; Fundamental and applied biological sciences. Psychology; Genetic aspects; genetic markers; Genetic polymorphisms; Genetics of eukaryotes. Biological and molecular evolution; Grasses; Hybridization; Identification and classification; Linkage (Genetics); linkage groups; loci; Morphology; Polyploidy; Pteridophyta, spermatophyta; restriction fragment length polymorphism; segregation; segregation distortion; tetraploidy; Vegetals; Zoysia; Zoysia japonica; Zoysia matrella; Japan; Genetic mapping; Monocotyledones; Linkage; Restriction fragment length polymorphism; Genetic marker; Gramineae; Angiospermae; Chromosome; Spermatophyta; Herbaceous plant; Zoysia
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051131

A RFLP linkage map of Zoysia spp. (2n = 40), a warm-season turfgrass, was constructed by using the self-pollinated progenies obtained from an interspecific hybrid. Out of 115 DNA clones tested, 100 (87.0%), including 55 genomic clones, 38 cDNA clones, and seven gene clones encoding photosynthetic enzymes showed allelic-RFLP banding patterns among the parental accessions. We found that 26 probes detected two or more loci segregating in the self-pollinated progenies independently. The RFLP linkage map of Zoysia spp. consists of 115 loci in 22 linkage groups ranging in size from 12.5 cM to 141.3 cM with a total map distance of 1506 cM. Six RFLP loci (5.4%) showed significant segregation distortion (P <0.01). Two loci out of six were mapped to linkage group II, and another two loci were mapped to group VII. In the RFLP linkage map of zoysiagrass, five pairs of linkage groups sharing a series of duplicated loci with approximately the same order were identified. Therefore, we conclude that Zoysia spp. with 2n = 40 should be considered as allotetraploids, which might have evolved from progenitors with a basic chromosome number of ten (x = 10).