Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis

To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin. Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the r...

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Published inActa cirurgica brasileira Vol. 29 Suppl 1; no. suppl 1; pp. 57 - 61
Main Authors Santos Filho, Ivan Dunshee de Abranches Oliveira, Enokihara, Milvia Maria Simões E Silva, Shie, Janaina Namba, Oliveira, Andrea Fernandes de, Santos, Ivan Dunshee de Abranches Oliveira, Ferreira, Lydia Masako
Format Journal Article
LanguageEnglish
Published Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2014
Subjects
Animals
Charcoal
Disease Models, Animal
Female
Frozen sections
Frozen Sections - methods
Lymph node biopsy
Lymph Nodes - pathology
Lymphatic Metastasis - pathology
Melanoma - pathology
Melanoma - secondary
Paraffin embedding
Paraffin Embedding - methods
Rats
Reference Standards
Reproducibility of Results
Sentinel Lymph Node Biopsy - methods
Time Factors
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Summary:To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin. Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were dissected and split longitudinally. One half of the lymph node was sent immediately to frozen section analysis. The other half was fixed in 10% formaldehyde to be cut in paraffin. Slides were divided into quadrants and classified by the presence of carbon in these four quadrants_ They were also classified by the carbon staining intensity. Comparing the slides obtained in the first day and 21 days, there was a tendency of carbon to spread over time, but without statistical significance. The intensity did not alter over time. There was no concordance between the two methods of pathological analysis, however the actived carbon was seen in all lymph nodes.
ISSN:0102-8650
1678-2674
0102-8650
DOI:10.1590/S0102-86502014001300011