In vitro iron absorption of α-lactalbumin hydrolysate-iron and β-lactoglobulin hydrolysate-iron complexes

• Published in: Journal of dairy science Vol. 97; no. 5; pp. 2559 - 2566
• Main Authors: Wang, X., Ai, T., Meng, X.L., Zhou, J., Mao, X.Y.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2014
• Subjects: Biological Transport; Caco-2 Cells; ferritin; Ferritins - metabolism; Humans; Iron; iron absorption; Lactalbumin - metabolism; Lactoglobulins - metabolism; Protein Hydrolysates - metabolism; α-lactalbumin hydrolysate-iron complex; β-lactoglobulin hydrolysate-iron complex; iron absorption; β-lactoglobulin hydrolysate-iron complex; ferritin; Caco-2 cells; α-lactalbumin hydrolysate-iron complex
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2013-7461

To study the feasibility of promoting iron absorption by peptides derived from α-lactalbumin and β-lactoglobulin, the present work examined the transport of iron across Caco-2 monolayer cell as in vitro model. Caco-2 cells were seeded in bicameral chambers with α-lactalbumin hydrolysate-Fe (α-LAH-Fe) complex and β-lactoglobulin hydrolysate-Fe (β-LGH-Fe) complex, α-LAH and iron mixture, β-LGH and iron mixture, FeSO4 and ascorbic acid mixture, and FeSO4. In addition, the cytotoxicity of α-LAH-Fe and β-LGH-Fe complexes were measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The iron absorption and ferritin content were assessed using the coupled in vitro digestion/Caco-2 cell model. Results support that peptide-iron complexes can promote ferritin formation and it is possible to apply β-LGH-Fe complexes as iron-fortified supplements with high iron absorbability.