Bioadhesive injectable hydrogel with phenolic carbon quantum dot supported Pd single atom nanozymes as a localized immunomodulation niche for cancer catalytic immunotherapy

• Published in: Biomaterials Vol. 280; p. 121272
• Main Authors: He, Huan, Fei, Ziying, Guo, Tailin, Hou, Yue, Li, Da, Wang, Kefeng, Ren, Fuzeng, Fan, Kelong, Zhou, Daijun, Xie, Chaoming, Wang, Chao, Lu, Xiong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.01.2022
• Subjects: Adhesive hydrogel; Carbon - therapeutic use; Humans; Hydrogels - pharmacology; Immunity; Immunomodulation; Immunotherapy; Injectable hydrogel; Neoplasms - drug therapy; Quantum Dots; Single atom nanozyme; Tumor Microenvironment; Adhesive hydrogel; Immunomodulation; Single atom nanozyme; Injectable hydrogel; Immunotherapy
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2021.121272

Immunotherapy is a powerful way to treat cancer, however, systemic treatment-associated adverse effects remain a major concern. In this study, a bioadhesive injectable hydrogel is developed to provide localized immune niches for tumor microenvironment immunomodulation and cancer catalytic immunotherapy. First, a phenolic single atom nanozyme (SAN) was developed by in situ synthesis of Pd single atom on catechol-grafted carbon-quantum-dot (DA-CQD@Pd) templates. Then, the bioadhesive injectable hydrogel consisting of DA-CQD@Pd SAN and immune adjuvant CpGODN was formed through SAN-catalyzed free-radical polymerization. The SAN exhibited peroxidase-like activity to generate ROS and kill tumor cells through catalytic therapy. The hydrogel locally released CpGODN in a sustained manner, which limited the risk of systemic exposure, reducing the impact of CpGODN toxicity, and protecting CpGODN from degradation. The bioadhesive hydrogel immobilized around solid tumor to provide an immune response site after injection. When combined it with the administration of immune checkpoint inhibitor anti-PD-L1, the hydrogel realized localized immunomodulation, maximized therapeutic efficacy and prevents tumor metastasis via a catalytic immunotherapy.