An adaptive teosinte mexicana introgression modulates phosphatidylcholine levels and is associated with maize flowering time

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 27; p. e2100036119
• Main Authors: Barnes, Allison C, Rodríguez-Zapata, Fausto, Juárez-Núñez, Karla A, Gates, Daniel J, Janzen, Garrett M, Kur, Andi, Wang, Li, Jensen, Sarah E, Estévez-Palmas, Juan M, Crow, Taylor M, Kavi, Heli S, Pil, Hannah D, Stokes, Ruthie L, Knizner, Kevan T, Aguilar-Rangel, Maria R, Demesa-Arévalo, Edgar, Skopelitis, Tara, Pérez-Limón, Sergio, Stutts, Whitney L, Thompson, Peter, Chiu, Yu-Chun, Jackson, David, Muddiman, David C, Fiehn, Oliver, Runcie, Daniel, Buckler, Edward S, Ross-Ibarra, Jeffrey, Hufford, Matthew B, Sawers, Ruairidh J H, Rellán-Álvarez, Rubén
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 05.07.2022
• Subjects: Adaptation, Physiological; Alleles; Amino acids; Biological Sciences; Chromosome Mapping; Conserved sequence; Corn; Flowering; Flowers - genetics; Flowers - metabolism; Gene mapping; Gene polymorphism; Gene-Environment Interaction; Genes, Plant; Genetic Linkage; Genomes; Genotype-environment interactions; Highlands; Lecithin; Lipid metabolism; Low temperature; Metabolites; Minority & ethnic groups; Mutagenesis; Native Americans; Phosphatidylcholine; Phosphatidylcholines - metabolism; Phospholipase A1; Phospholipases A1 - classification; Phospholipases A1 - genetics; Phospholipases A1 - metabolism; Phospholipids; Phosphorus; Plant breeding; Plant Proteins - classification; Plant Proteins - genetics; Plant Proteins - metabolism; Polymorphism; Prokaryotes; Quantitative trait loci; Zea luxurians; Zea mays; Zea mays - genetics; Zea mays - growth & development; phospholipid metabolism; highland adaptation; maize genetics; selection; flowering time
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2100036119

Native Americans domesticated maize ( ssp. ) from lowland teosinte ( ssp. in the warm Mexican southwest and brought it to the highlands of Mexico and South America where it was exposed to lower temperatures that imposed strong selection on flowering time. Phospholipids are important metabolites in plant responses to low-temperature and phosphorus availability and have been suggested to influence flowering time. Here, we combined linkage mapping with genome scans to identify ( ), a gene that encodes a phospholipase A1 enzyme, as a major driver of phospholipid variation in highland maize. Common garden experiments demonstrated strong genotype-by-environment interactions associated with variation at with the highland allele leading to higher fitness in highlands, possibly by hastening flowering. The highland maize variant resulted in impaired function of the encoded protein due to a polymorphism in a highly conserved sequence. A meta-analysis across HPC1 orthologs indicated a strong association between the identity of the amino acid at this position and optimal growth in prokaryotes. Mutagenesis of via genome editing validated its role in regulating phospholipid metabolism. Finally, we showed that the highland allele entered cultivated maize by introgression from the wild highland teosinte ssp. and has been maintained in maize breeding lines from the Northern United States, Canada, and Europe. Thus, introgressed from teosinte underlies a large metabolic QTL that modulates phosphatidylcholine levels and has an adaptive effect at least in part via induction of early flowering time.