Anti-inflammatory effect of heme oxygenase-1 toward Porphyromonas gingivalis lipopolysaccharide in macrophages exposed to gomisins A, G, and J

• Published in: Journal of medicinal food Vol. 14; no. 12; p. 1519
• Main Authors: Ryu, Eun Yeon, Park, Sun Young, Kim, Sun Gun, Park, Da Jung, Kang, Jum Soon, Kim, Young Hun, Seetharaman, Rajaseker, Choi, Young-Whan, Lee, Sang-Joon
• Format: Journal Article
• Language: English
• Published: United States 01.12.2011
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Cell Line; Cyclooctanes - pharmacology; Dioxoles - pharmacology; Fruit - chemistry; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; Inflammation - metabolism; Interleukin-1beta - antagonists & inhibitors; Interleukin-1beta - biosynthesis; Interleukin-6 - antagonists & inhibitors; Interleukin-6 - biosynthesis; Lignans - pharmacology; Lipopolysaccharides - pharmacology; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; Plant Extracts - pharmacology; Polycyclic Compounds - pharmacology; Porphyromonas gingivalis - pathogenicity; Schisandra - chemistry; Signal Transduction; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - biosynthesis
• ISSN: 1557-7600
• DOI: 10.1089/jmf.2011.1656

Periodontitis, a chronic inflammatory periodontal disease that develops from gingivitis, is caused by periodontal pathogenic bacteria such as Porphyromonas gingivalis. Recent studies have focused on the antioxidant, anti-human immunodeficiency virus, anticarcinogenic, and anti-inflammatory properties of gomisins. However, the anti-inflammatory activities of gomisin plants through heme oxygenase-1 (HO-1) signals remain poorly defined. We found that gomisins' anti-inflammatory activity occurs via the induction of HO-1 expression. Gomisins G and J inhibit the production of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 and also block nuclear factor-κB activation in Raw264.7 cells stimulated with P. gingivalis lipopolysaccharide. Furthermore, pro-inflammatory cytokine production is inhibited through the induction of HO-1 expression. HO-1 expression is induced by all gomisins, but their anti-inflammatory activity via HO-1 signaling is observed with gomisins G and J, and not A. We found that gomisins G and J extracted from Schisandria chinensis can inhibit the P. gingivalis lipopolysaccharide induced-inflammatory responses in Raw264.7 cells.