The histidine residues in pig and horse colipases

The single histidine of horse colipase B was shown to be at position 29, thus definitely proving that His 86 present in the pig cofactor is not conserved in the horse. Carbethoxylation of histidines did not appreciably affect the capacity of the porcine cofactor to bind to hydrophobic interfaces and...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 99; no. 1; pp. 114 - 119
Main Authors Granon, Simone, Rahmani-Jourdheuil, Dominique, Desnuelle, Pierre, Chapus, Catherine
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 16.03.1981
Subjects
Amino Acid Sequence
Animals
Chemical Phenomena
Chemistry
Colipases - physiology
Histidine
Horses
Proteins - physiology
Swine
CNBr
DEPC
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Summary:The single histidine of horse colipase B was shown to be at position 29, thus definitely proving that His 86 present in the pig cofactor is not conserved in the horse. Carbethoxylation of histidines did not appreciably affect the capacity of the porcine cofactor to bind to hydrophobic interfaces and to anchor lipase in presence of bile salts, but it induced in the aromatic region of the molecule a significant transconformation detectable by spectrofluorimetry.
Bibliography:L
L50
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(81)91720-4