Capillary Electrophoresis with Laser Induced Fluorescence Detection for Study of the Association of HSP60 Gene Polymorphism with Gouty Arthritis

• Published in: Journal of AOAC International Vol. 102; no. 3; pp. 810 - 814
• Main Authors: Ren, Dongxia, Fan, Min, Sun, Chengjun, Zhou, Chen, Li, Yongxin
• Format: Journal Article
• Language: English
• Published: England AOAC International 01.05.2019
• Subjects: Adult; Arthritis; Arthritis, Gouty - genetics; Backup software; Chaperonin 60 - genetics; Deoxyribonucleases, Type II Site-Specific - chemistry; DNA; DNA - analysis; DNA - genetics; EDTA; Electrophoresis; Electrophoresis, Capillary - methods; Female; Fluorescence; Genes; Genetic aspects; Genetic polymorphisms; Gout; Health; Health aspects; Heat shock proteins; Humans; Male; Middle Aged; Mitochondrial Proteins - genetics; Nucleases; Nucleic acids; Polymorphism, Genetic; Regression Analysis; Risk factors
• ISSN: 1060-3271; 1944-7922; 1944-7922
• DOI: 10.5740/jaoacint.18-0242

Background: Gout arthritis is a common inflammatory arthritis and it poses a major threat to human health. Objective: A method of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the detection of HSP60 gene polymorphism has been developed and applied to the exploration of correlation between gouty arthritis and HSP60 gene polymorphism. Methods: The genomic deoxyribonucleic acid from 59 patients with gouty arthritis and 64 control subjects was extracted and the conservative fragment of HSP60 was amplified. The products were digested with restriction endonuclease NlaIII and then separated and detected by the proposed method. Results: In the case group, there were 7 cases of TT genotype, 29 cases of CC genotype, and 23 cases of CT genotype. In the control group, there were 4 cases of TT genotype, 6 cases of CC genotype, and 54 cases of CT genotype. The detection results of the samples were statistically analyzed by binary logistic regression and Spearman correlation analysis. After adjusting gender, age, and other compounding factors, the TT genotype and CT genotype of the HSP60 gene were found to affect gouty arthritis. Conclusions: When used for gene polymorphism research, the proposed CE-LIF method has the advantages of high efficiency, rapidity, sensitivity, and low sample consumption. A moderate correlation between gouty arthritis and HSP60 genotype distribution was discovered for the first time. Highlights: A new method using CE-LIF for the detection of HSP60 gene polymorphism of 59 patients with gouty arthritis and 64 control subjects in China. The correlation between gouty arthritis and HSP60 gene polymorphism was explored for the first time.