Isolation of novel early salt-responding genes from wheat (Triticum aestivum L.) by differential display

• Published in: Theoretical and applied genetics Vol. 98; no. 5; pp. 673 - 678
• Main Authors: Nemoto, Y, Kawakami, N, Sasakuma, T
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.04.1999; Berlin Springer Nature B.V
• Subjects: Abiotic stress; amino acid sequences; Antisense DNA; Biological and medical sciences; Cloning; complementary DNA; Fundamental and applied biological sciences. Psychology; genbank/ab011441; genbank/ab011442; genbank/ab011443; genbank/ab011444; genbank/ab011445; Gene expression; Genes. Genome; Genetic aspects; glucose-6-phosphate 1-dehydrogenase; Identification and classification; leaves; messenger RNA; Molecular and cellular biology; Molecular genetics; molecular sequence data; nucleotide sequences; Phenols; Plant genetics; Plant growth; plant proteins; polypeptides; roots; Salinity; Salt; Salt stress (Botany); sodium chloride; stress; Toxicity; transcription factors; Triticum aestivum; Wheat; Japan; Monocotyledones; Glucose-6-phosphate 1-dehydrogenase; Salt resistance; Nucleotide sequence; Enzyme; Induction; Zinc finger structure; Homology; Gene expression; Cereal crop; Stress; Osmotic shock; Regulation(control); Complementary DNA; Gramineae; Angiospermae; Spermatophyta; Oxidoreductases; Triticum aestivum; Sodium Chlorides
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051120

We isolated five cDNAs for salt-stress responding genes from common wheat, Triticum aestivum L., seedlings treated with 0.15 M NaCl for 2 h by using the modified differential display method. The clones were collectively named WESR (Wheat Early Salt-stress Responding) genes. WESR1 to 3 were isolated from leaves, and WESR4 and 5, from roots. Induction of the gene expression by NaCl treatment for 2 h was confirmed by Northern blot analysis. WESR gene transcripts accumulated from 1.3 to 2.6-fold over the controls. Based on nucleotide and deduced amino acid sequence analysis. WESR4 showed homology to a cDNA clone isolated from barley containing the zinc-finger motif. The deduced amino acid sequence of WESR5 indicated homology to the glucose-6-phosphate dehydrogenase (G6PDH) reported in potato and alfalfa. Other WESR clones did not show homology to any genes of known function, and all WESR clones are novel salt-stress responding genes.