Ferredoxin biosynthesis in Euglena gracilis

• Published in: Biochimica et biophysica acta Vol. 442; no. 1; pp. 76 - 87
• Main Authors: Matson, R.S, Kimura, T
• Format: Journal Article
• Language: English
• Published: Netherlands 02.08.1976
• Subjects: Animals; Biological Transport, Active; Cell Division - drug effects; Chloramphenicol - metabolism; Chloramphenicol - pharmacology; Chlorophyll - metabolism; Cycloheximide - pharmacology; Cytoplasm - metabolism; Erythromycin - pharmacology; Euglena gracilis - drug effects; Euglena gracilis - metabolism; Ferredoxins - biosynthesis; Glucosephosphate Dehydrogenase - metabolism; Iron - metabolism; plant biochemistry; plant physiology; Ribosomes - drug effects; Ribosomes - metabolism; Rifampin - pharmacology
• ISSN: 0006-3002; 1878-2434
• DOI: 10.1016/0005-2787(76)90178-7

Analysis of ferredoxin content in cultures of Euglena gracilis grown in the presence of selective antibiotic inhibitors of protein synthesis resulted in the following conclusions: 1. Ferredoxin is synthesized from cytoplasmic (80s-type) ribosomes; cycloheximide, a potent inhibitor of 80s translation completely abolished the synthesis, while the inhibitors of 70s translation chloramphenicol and erythromycin were not effective. In addition, ferredoxin was detected in a streptomycin-bleached mutant that lacks the chloroplast structure and chloroplast DNA. 2. Ferredoxin's transcript is presumably of nuclear origin; rifampicin, an inhibitor of chloroplast DNA-dependent RNA polymerase did not inhibit synthesis, while the streptomycin-bleached mutant continued to synthesize ferredoxin without chloroplast DNA.